Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Metabolic engineering of low-pH-tolerant non-model yeast, Issatchenkia orientalis, for production of citramalate

Journal Article · · Metabolic Engineering Communications
 [1];  [2];  [3];  [3];  [4];  [5];  [5];  [3];  [4];  [6];  [7]
  1. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of Illinois
  2. Iowa State University, Ames, IA (United States); University of Illinois at Urbana-Champaign, IL (United States)
  3. Princeton University, NJ (United States)
  4. Pennsylvania State University, University Park, PA (United States)
  5. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
  6. Iowa State University, Ames, IA (United States); Ames Laboratory (AMES), Ames, IA (United States); University of Illinois at Urbana-Champaign, IL (United States)
  7. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Hokkaido University (Japan)
+ Show Author Affiliations
Methyl methacrylate (MMA) is an important petrochemical with many applications. However, its manufacture has a large environmental footprint. Combined biological and chemical synthesis (semisynthesis) may be a promising alternative to reduce both cost and environmental impact, but strains that can produce the MMA precursor (citramalate) at low pH are required. A non-conventional yeast, Issatchenkia orientalis, may prove ideal, as it can survive extremely low pH. Here, we demonstrate the engineering of I. orientalis for citramalate pro- duction. Using sequence similarity network analysis and subsequent DNA synthesis, we selected a more active citramalate synthase gene (cimA) variant for expression in I. orientalis. We then adapted a piggyBac transposon system for I. orientalis that allowed us to simultaneously explore the effects of different cimA gene copy numbers and integration locations. A batch fermentation showed the genome-integrated-cimA strains produced 2.0 g/L citramalate in 48 h and a yield of up to 7% mol citramalate/mol consumed glucose. These results demonstrate the potential of I. orientalis as a chassis for citramalate production.
Research Organization:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231; SC0018420
OSTI ID:
1993839
Journal Information:
Metabolic Engineering Communications, Journal Name: Metabolic Engineering Communications Vol. 16; ISSN 2214-0301
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

References (38)

Manipulating Natural Product Biosynthetic Pathways via DNA Assembler journal June 2014
Developing a piggyBac Transposon System and Compatible Selection Markers for Insertional Mutagenesis and Genome Engineering in Yarrowia lipolytica journal March 2018
Production of citramalate by metabolically engineered Escherichia coli: Citramalate Production in Escherichia coli journal September 2016
Low‐pH production of d ‐lactic acid using newly isolated acid tolerant yeast Pichia kudriavzevii NG7 journal June 2018
Yeast myosin heavy chain mutant: Maintenance of the cell type specific budding pattern and the normal deposition of chitin and cell wall components requires an intact myosin heavy chain gene journal January 1990
Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae journal October 2009
DNA Assembler Method for Construction of Zeaxanthin-Producing Strains of Saccharomyces cerevisiae book January 2012
Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks journal August 2015
Low pH d-xylonate production with Pichia kudriavzevii journal April 2013
Metabolic engineering of an acid-tolerant yeast strain Pichia kudriavzevii for itaconic acid production journal June 2020
Genome-scale metabolic reconstruction of the non-model yeast Issatchenkia orientalis SD108 and its application to organic acids production journal December 2020
A genetic toolbox for metabolic engineering of Issatchenkia orientalis journal May 2020
Sustainable Production of Acrylic Acid via 3-Hydroxypropionic Acid from Lignocellulosic Biomass journal November 2021
Leveraging the Hermes Transposon to Accelerate the Development of Nonconventional Yeast-based Microbial Cell Factories journal May 2020
Streamlining the Design-to-Build Transition with Build-Optimization Software Tools journal November 2016
Rewriting yeast central carbon metabolism for industrial isoprenoid production journal September 2016
A cross-platform toolkit for mass spectrometry and proteomics journal October 2012
Synonymous but not the same: the causes and consequences of codon bias journal November 2010
Catalysis for the synthesis of methacrylic acid and methyl methacrylate journal January 2018
Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae journal February 2007
A hyperactive piggyBac transposase for mammalian applications journal January 2011
Citramalate synthase yields a biosynthetic pathway for isoleucine and straight- and branched-chain ester formation in ripening apple fruit journal January 2021
MycoCosm portal: gearing up for 1000 fungal genomes journal December 2013
IMG/M v.5.0: an integrated data management and comparative analysis system for microbial genomes and microbiomes journal October 2018
Efficient bio-production of citramalate using an engineered Escherichia coli strain journal February 2018
Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks journal November 2003
Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae journal January 2012
Elucidation of an Alternate Isoleucine Biosynthesis Pathway in Geobacter sulfurreducens journal February 2008
( R )-Citramalate Synthase in Methanogenic Archaea journal January 1999
Nonhomologous-End-Joining Factors Regulate DNA Repair Fidelity during Sleeping Beauty Element Transposition in Mammalian Cells journal December 2003
Microhomology-Dependent End Joining and Repair of Transposon-Induced DNA Hairpins by Host Factors in Saccharomyces cerevisiae journal February 2004
Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast journal November 2014
Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis journal June 2019
Exploiting Issatchenkia orientalis SD108 for succinic acid production journal August 2014
Screening of Metagenomic and Genomic Libraries Reveals Three Classes of Bacterial Enzymes That Overcome the Toxicity of Acrylate journal May 2014
PMMA: An Essential Material in Medicine and Dentistry journal January 2005
A Review of the Biotechnological Production of Methacrylic Acid journal March 2020
Prosthodontic Applications of Polymethyl Methacrylate (PMMA): An Update journal October 2020

Similar Records

Data for Metabolic Engineering of Low-pH-Tolerant Non-Model Yeast, Issatchenkia orientalis, for Production of Citramalate
Dataset · Wed Feb 15 19:00:00 EST 2023 · OSTI ID:3014474
A transcriptomic atlas of acute stress response to low pH in multiple Issatchenkia orientalis strains
Journal Article · Tue Nov 28 19:00:00 EST 2023 · Microbiology Spectrum · OSTI ID:2309742
Cas9-Based Metabolic Engineering of Issatchenkia orientalis for Enhanced Utilization of Cellulosic Hydrolysates
Journal Article · Tue Sep 13 20:00:00 EDT 2022 · Journal of Agricultural and Food Chemistry · OSTI ID:1902741

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Acid tolerance
Citramalate
Issatchenkia orientalis
Poly(methyl methacrylate) (PMMA)
Transposon