Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling

Suthers, Patrick F.; Maranas, Costas D.

doi:10.1002/btpr.3276

Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling

Journal Article · Tue Jun 28 00:00:00 EDT 2022 · Biotechnology Progress

DOI:https://doi.org/10.1002/btpr.3276· OSTI ID:1874060

Suthers, Patrick F. ^[1]; ^[1]

Department of Chemical Engineering The Pennsylvania State University University Park Pennsylvania USA, Center for Advanced Bioenergy and Bioproducts Innovation The Pennsylvania State University University Park Pennsylvania USA

Abstract

Growth‐coupling product formation can facilitate strain stability by aligning industrial objectives with biological fitness. Organic acids make up many building block chemicals that can be produced from sugars obtainable from renewable biomass. Issatchenkia orientalis is a yeast strain tolerant to acidic conditions and is thus a promising host for industrial production of organic acids. Here, we use constraint‐based methods to assess the potential of computationally designing growth‐coupled production strains for I. orientalis that produce 22 different organic acids under aerobic or microaerobic conditions. We explore native and engineered pathways using glucose or xylose as the carbon substrates as proxy constituents of hydrolyzed biomass. We identified growth‐coupled production strategies for 37 of the substrate‐product pairs, with 15 pairs achieving production for any growth rate. We systematically assess the strain design solutions and categorize the underlying principles involved.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018260; SC0018420

OSTI ID:: 1874060

Alternate ID(s):: OSTI ID: 1874061
OSTI ID: 1902714

Journal Information:: Biotechnology Progress, Journal Name: Biotechnology Progress Journal Issue: 5 Vol. 38; ISSN 8756-7938

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United States

Language:: English

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constraint-based modeling
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nonmodel yeast

Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling

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