Data for L-Malic Acid Production from Xylose by Engineered Saccharomyces cerevisiae

Kang, Nam Kyu; Lee, Jaewon; Ort, Donald; Jin, Yong-Su

doi:10.13012/B2IDB-7864158_V1

Title: Data for L-Malic Acid Production from Xylose by Engineered Saccharomyces cerevisiae

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Abstract

L-malic acid is widely used in the food, chemical, and pharmaceutical industries. Here, we report on production of malic acid from xylose, the second most abundant sugar in lignocellulosic hydrolysates, by engineered Saccharomyces cerevisiae. To enable malic acid production in a xylose-assimilating S. cerevisiae, we overexpressed PYC1 and PYC2, coding for pyruvate carboxylases, a truncated MDH3 coding for malate dehydrogenase, and SpMAE1, coding for a Schizosaccharomyces pombe malate transporter. Additionally, both the ethanol- and glycerol-producing pathways were blocked to enhance malic acid production. The resulting strain produced malic acid from both glucose and xylose, but it produced much higher titers of malic acid from xylose than glucose. Interestingly, the engineered strain had higher malic acid yield from lower concentrations (10 g L‒1) of xylose, with no ethanol production, than from higher xylose concentrations (20 and 40 g L‒1). As such, a fed-batch culture maintaining xylose concentrations at low levels was conducted and 61.2 g L‒1 of malic acid was produced, with a productivity of 0.32 g L‒1 h. These results represent successful engineering of S. cerevisiae for the production of malic acid from xylose, confirming that that xylose offers the efficient production of various biofuels and chemicals by engineered S.more » cerevisiae.« less

Authors:

; Lee, Jaewon;

; Jin, Yong-Su

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; Departments of Plant Biology and Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Publication Date:: Fri Aug 13 00:00:00 UTC 2021

DOE Contract Number:: SC0018420

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign

Sponsoring Org.:: U.S. Department of Energy (DOE)

Subject:: Conversion; Feedstock Production; Genome Engineering

OSTI Identifier:: 3014148

DOI:: https://doi.org/10.13012/B2IDB-7864158_V1

Citation Formats


                    Kang, Nam Kyu, Lee, Jaewon, Ort, Donald, and Jin, Yong-Su. Data for L-Malic Acid Production from Xylose by Engineered Saccharomyces cerevisiae.  United States: N. p., 2021. 
        Web.  doi:10.13012/B2IDB-7864158_V1.

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                    Kang, Nam Kyu, Lee, Jaewon, Ort, Donald, & Jin, Yong-Su. Data for L-Malic Acid Production from Xylose by Engineered Saccharomyces cerevisiae.  United States.  doi:https://doi.org/10.13012/B2IDB-7864158_V1

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                    Kang, Nam Kyu, Lee, Jaewon, Ort, Donald, and Jin, Yong-Su. 2021.  
"Data for L-Malic Acid Production from Xylose by Engineered Saccharomyces cerevisiae".  United States.  doi:https://doi.org/10.13012/B2IDB-7864158_V1.  https://www.osti.gov/servlets/purl/3014148. Pub date:Fri Aug 13 00:00:00 UTC 2021

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@article{osti_3014148,

  title        = {Data for L-Malic Acid Production from Xylose by Engineered Saccharomyces cerevisiae},

  author       = {Kang, Nam Kyu and Lee, Jaewon and Ort, Donald and Jin, Yong-Su},

  abstractNote = {L-malic acid is widely used in the food, chemical, and pharmaceutical industries. Here, we report on production of malic acid from xylose, the second most abundant sugar in lignocellulosic hydrolysates, by engineered Saccharomyces cerevisiae. To enable malic acid production in a xylose-assimilating S. cerevisiae, we overexpressed PYC1 and PYC2, coding for pyruvate carboxylases, a truncated MDH3 coding for malate dehydrogenase, and SpMAE1, coding for a Schizosaccharomyces pombe malate transporter. Additionally, both the ethanol- and glycerol-producing pathways were blocked to enhance malic acid production. The resulting strain produced malic acid from both glucose and xylose, but it produced much higher titers of malic acid from xylose than glucose. Interestingly, the engineered strain had higher malic acid yield from lower concentrations (10 g L‒1) of xylose, with no ethanol production, than from higher xylose concentrations (20 and 40 g L‒1). As such, a fed-batch culture maintaining xylose concentrations at low levels was conducted and 61.2 g L‒1 of malic acid was produced, with a productivity of 0.32 g L‒1 h. These results represent successful engineering of S. cerevisiae for the production of malic acid from xylose, confirming that that xylose offers the efficient production of various biofuels and chemicals by engineered S. cerevisiae.},

  doi          = {10.13012/B2IDB-7864158_V1},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Aug 13 00:00:00 UTC 2021},

  month        = {Fri Aug 13 00:00:00 UTC 2021}

}

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DOI: https://doi.org/10.13012/B2IDB-7864158_V1

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