Data for High-Level β-Carotene Production from Xylose by Engineered Saccharomyces cerevisiae without Overexpression of a Truncated HMG1 (tHMG1)

Sun, Liang; Atkinson, Christine A.; Lee, Ye-Gi; Jin, Yong-Su

doi:10.13012/B2IDB-4573999_V1

Title: Data for High-Level β-Carotene Production from Xylose by Engineered Saccharomyces cerevisiae without Overexpression of a Truncated HMG1 (tHMG1)

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Abstract

β‐Carotene is a natural pigment and health‐promoting metabolite, and has been widely used in the nutraceutical, feed, and cosmetic industries. Here, we engineered a GRAS yeast Saccharomyces cerevisiae to produce β‐carotene from xylose, the second most abundant and inedible sugar component of lignocellulose biomass. Specifically, a β‐carotene biosynthetic pathway containing crtYB, crtI, and crtE from Xanthophyllomyces dendrorhous was introduced into a xylose‐fermenting S. cerevisiae. The resulting strain produced β‐carotene from xylose at a titer threefold higher than from glucose. Interestingly, overexpression of tHMG1, which has been reported as a critical genetic perturbation to enhance metabolic fluxes in the mevalonate pathway and β‐carotene production in yeast when glucose is used, did not further improve the production of β‐carotene from xylose. Through fermentation profiling, metabolites analysis, and transcriptional studies, we found the advantages of using xylose as a carbon source, instead of glucose, for β‐carotene production to be a more respiratory feature of xylose consumption, a larger cytosolic acetyl‐CoA pool, and an upregulated expression level of rate‐limiting genes in the β‐carotene‐producing pathway, including ACS1 and HMG1. As a result, 772.8 mg/L of β‐carotene was obtained in a fed‐batch bioreactor culture with xylose feeding. Considering the inevitable large scale production of xylose when cellulosicmore »« less

Authors:

Sun, Liang; Atkinson, Christine A.; Lee, Ye-Gi; Jin, Yong-Su

Department of Food Science and Human Nutrition, University of Illinois at Urbana‐Champaign, Urbana, Illinois; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana‐Champaign, Urbana, Illinois; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Department of Food Science and Human Nutrition, University of Illinois at Urbana‐Champaign, Urbana, Illinois; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana‐Champaign, Urbana, Illinois

Publication Date:: Fri Jul 17 00:00:00 UTC 2020

DOE Contract Number:: SC0018420

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign

Sponsoring Org.:: U.S. Department of Energy (DOE)

Subject:: Conversion; Genome Engineering

OSTI Identifier:: 3013838

DOI:: https://doi.org/10.13012/B2IDB-4573999_V1

Citation Formats


                    Sun, Liang, Atkinson, Christine A., Lee, Ye-Gi, and Jin, Yong-Su. Data for High-Level β-Carotene Production from Xylose by Engineered Saccharomyces cerevisiae without Overexpression of a Truncated HMG1 (tHMG1).  United States: N. p., 2020. 
        Web.  doi:10.13012/B2IDB-4573999_V1.

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                    Sun, Liang, Atkinson, Christine A., Lee, Ye-Gi, & Jin, Yong-Su. Data for High-Level β-Carotene Production from Xylose by Engineered Saccharomyces cerevisiae without Overexpression of a Truncated HMG1 (tHMG1).  United States.  doi:https://doi.org/10.13012/B2IDB-4573999_V1

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                    Sun, Liang, Atkinson, Christine A., Lee, Ye-Gi, and Jin, Yong-Su. 2020.  
"Data for High-Level β-Carotene Production from Xylose by Engineered Saccharomyces cerevisiae without Overexpression of a Truncated HMG1 (tHMG1)".  United States.  doi:https://doi.org/10.13012/B2IDB-4573999_V1.  https://www.osti.gov/servlets/purl/3013838. Pub date:Fri Jul 17 00:00:00 UTC 2020

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@article{osti_3013838,

  title        = {Data for High-Level β-Carotene Production from Xylose by Engineered Saccharomyces cerevisiae without Overexpression of a Truncated HMG1 (tHMG1)},

  author       = {Sun, Liang and Atkinson, Christine A. and Lee, Ye-Gi and Jin, Yong-Su},

  abstractNote = {β‐Carotene is a natural pigment and health‐promoting metabolite, and has been widely used in the nutraceutical, feed, and cosmetic industries. Here, we engineered a GRAS yeast Saccharomyces cerevisiae to produce β‐carotene from xylose, the second most abundant and inedible sugar component of lignocellulose biomass. Specifically, a β‐carotene biosynthetic pathway containing crtYB, crtI, and crtE from Xanthophyllomyces dendrorhous was introduced into a xylose‐fermenting S. cerevisiae. The resulting strain produced β‐carotene from xylose at a titer threefold higher than from glucose. Interestingly, overexpression of tHMG1, which has been reported as a critical genetic perturbation to enhance metabolic fluxes in the mevalonate pathway and β‐carotene production in yeast when glucose is used, did not further improve the production of β‐carotene from xylose. Through fermentation profiling, metabolites analysis, and transcriptional studies, we found the advantages of using xylose as a carbon source, instead of glucose, for β‐carotene production to be a more respiratory feature of xylose consumption, a larger cytosolic acetyl‐CoA pool, and an upregulated expression level of rate‐limiting genes in the β‐carotene‐producing pathway, including ACS1 and HMG1. As a result, 772.8 mg/L of β‐carotene was obtained in a fed‐batch bioreactor culture with xylose feeding. Considering the inevitable large scale production of xylose when cellulosic biomass‐based bioeconomy is implemented, our results suggest xylose utilization is a promising strategy for overproduction of carotenoids and other isoprenoids in engineered S. cerevisiae.},

  doi          = {10.13012/B2IDB-4573999_V1},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Jul 17 00:00:00 UTC 2020},

  month        = {Fri Jul 17 00:00:00 UTC 2020}

}

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DOI: https://doi.org/10.13012/B2IDB-4573999_V1

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