Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans

Noguera, Daniel R.; Donohue, Timothy J.

doi:10.82250/3003593

Title: Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans

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Abstract

This study investigates the catabolism of the b-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidates elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A to aromatic monomers that can be funneled into the central aromatic metabolic pathway of N. aromaticivorans.

Authors:

Noguera, Daniel R.; Donohue, Timothy J.

GLBRC - University of Wisconsin

Publication Date:: Thu Feb 29 04:00:00 UTC 2024

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: aromatic compounds; genetics; genomics; lininolysis

OSTI Identifier:: 3003593

DOI:: https://doi.org/10.82250/3003593

Citation Formats


                    Noguera, Daniel R., and Donohue, Timothy J. Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans.  United States: N. p., 2024. 
        Web.  doi:10.82250/3003593.

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                    Noguera, Daniel R., & Donohue, Timothy J. Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans.  United States.  doi:https://doi.org/10.82250/3003593

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                    Noguera, Daniel R., and Donohue, Timothy J. 2024.  
"Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans".  United States.  doi:https://doi.org/10.82250/3003593.  https://www.osti.gov/servlets/purl/3003593. Pub date:Thu Feb 29 04:00:00 UTC 2024

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@article{osti_3003593,

  title        = {Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans},

  author       = {Noguera, Daniel R. and Donohue, Timothy J.},

  abstractNote = {This study investigates the catabolism of the b-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidates elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A to aromatic monomers that can be funneled into the central aromatic metabolic pathway of N. aromaticivorans.},

  doi          = {10.82250/3003593},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Feb 29 04:00:00 UTC 2024},

  month        = {Thu Feb 29 04:00:00 UTC 2024}

}

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DOI: https://doi.org/10.82250/3003593

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Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans

Dataset Noguera, Daniel R. ; Donohue, Timothy J.

This study investigates the catabolism of the b-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidates elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A to aromatic monomers that can be funneled into the central aromatic metabolic pathway of N. aromaticivorans.
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Catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans

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ABSTRACT Aromatic compounds are an important source of commodity chemicals traditionally produced from fossil fuels. Aromatics derived from plant lignin can potentially be converted into commodity chemicals through depolymerization followed by microbial funneling of monomers and low molecular weight oligomers. This study investigates the catabolism of the β-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans . We used genome-wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidate genes elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convertmore »« less
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ABSTRACT Aromatic compounds are an important source of commodity chemicals traditionally produced from fossil fuels. Aromatics derived from plant lignin can potentially be converted into commodity chemicals through depolymerization followed by microbial funneling of monomers and low molecular weight oligomers. This study investigates the catabolism of the β-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans . We used genome-wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidate genes elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convertmore »« less
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Aromatic compounds are an important source of commodity chemicals traditionally produced from fossil fuels. Aromatics derived from plant lignin can potentially be converted into commodity chemicals through depolymerization followed by microbial funneling of monomers and low molecular weight oligomers. This study investigates the catabolism of the β-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome-wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidate genes elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A tomore »« less

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