U.S. Department of EnergyOffice of Scientific and Technical Information
Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans
Abstract
This study investigates the catabolism of the b-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidates elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A to aromatic monomers that can be funneled into the central aromatic metabolic pathway of N. aromaticivorans.
- Authors:
- Publication Date:
- DOE Contract Number:
- SC0018409
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC) (GLBRC)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 3001519
- DOI:
- https://doi.org/10.82250/3001519
Citation Formats
Noguera, Daniel R., and Donohue, Timothy J. Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans. United States: N. p., 2024.
Web. doi:10.82250/3001519.
Noguera, Daniel R., & Donohue, Timothy J. Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans. United States. doi:https://doi.org/10.82250/3001519
Noguera, Daniel R., and Donohue, Timothy J. 2024.
"Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans". United States. doi:https://doi.org/10.82250/3001519. https://www.osti.gov/servlets/purl/3001519. Pub date:Wed Feb 28 23:00:00 EST 2024
@article{osti_3001519,
title = {Tnseq to identify catabolism of b-5 linked aromatics by Novosphingobium aromaticivorans},
author = {Noguera, Daniel R. and Donohue, Timothy J.},
abstractNote = {This study investigates the catabolism of the b-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidates elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A to aromatic monomers that can be funneled into the central aromatic metabolic pathway of N. aromaticivorans.},
doi = {10.82250/3001519},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Feb 28 23:00:00 EST 2024},
month = {Wed Feb 28 23:00:00 EST 2024}
}
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