RNA-seq studying the catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans

Donohue, Timothy J.; Metz, Fletcher

doi:10.82250/3003592

Title: RNA-seq studying the catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans

Dataset
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Abstract

RNA-seq comparison of transcript abundance in a PDC-producing N. aromaticivorans strain (12444PDC) grown in the presence of dehydrodiconiferyl alcohol (DC-A) and glucose, vanillin and glucose, ferulic acid and glucose, or glucose alone

Authors:

Donohue, Timothy J.; Metz, Fletcher

GLBRC - University of Wisconsin
GLBRC - University of Wisconsin-Madison

Publication Date:: Thu Feb 29 04:00:00 UTC 2024

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: DC-A; RNA-seq; aromatics; lignin

OSTI Identifier:: 3003592

DOI:: https://doi.org/10.82250/3003592

Citation Formats


                    Donohue, Timothy J., and Metz, Fletcher. RNA-seq studying the catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans.  United States: N. p., 2024. 
        Web.  doi:10.82250/3003592.

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                    Donohue, Timothy J., & Metz, Fletcher. RNA-seq studying the catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans.  United States.  doi:https://doi.org/10.82250/3003592

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                    Donohue, Timothy J., and Metz, Fletcher. 2024.  
"RNA-seq studying the catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans".  United States.  doi:https://doi.org/10.82250/3003592.  https://www.osti.gov/servlets/purl/3003592. Pub date:Thu Feb 29 04:00:00 UTC 2024

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@article{osti_3003592,

  title        = {RNA-seq studying the catabolism of β-5 linked aromatics by Novosphingobium aromaticivorans},

  author       = {Donohue, Timothy J. and Metz, Fletcher},

  abstractNote = {RNA-seq comparison of transcript abundance in a PDC-producing N. aromaticivorans strain (12444PDC) grown in the presence of dehydrodiconiferyl alcohol (DC-A) and glucose, vanillin and glucose, ferulic acid and glucose, or glucose alone},

  doi          = {10.82250/3003592},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Feb 29 04:00:00 UTC 2024},

  month        = {Thu Feb 29 04:00:00 UTC 2024}

}

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DOI: https://doi.org/10.82250/3003592

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Aromatic compounds are an important source of commodity chemicals traditionally produced from fossil fuels. Aromatics derived from plant lignin can potentially be converted into commodity chemicals through depolymerization followed by microbial funneling of monomers and low molecular weight oligomers. This study investigates the catabolism of the β-5 linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) by the bacterium Novosphingobium aromaticivorans. We used genome-wide screens to identify candidate genes involved in DC-A catabolism. Subsequent in vivo and in vitro analyses of these candidate genes elucidated a catabolic pathway composed of four required gene products and several partially redundant dehydrogenases that convert DC-A tomore »« less

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