PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5)

Anderson, Lindsey N; Kim, Young-Mo; Waters, Katrina M; Bohutskyi, Pavlo

doi:10.25584/2510499

Title: PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5)

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Abstract

The purpose of this experiment was to evaluate how circadian clock regulation impacts carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 in providing insights to strategies for enhanced bioproduction. Culture samples were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours for extracellular sucrose analysis. Circadian metabolomics data was acquired using a Agilent single quadrupole gas chromatography-mass spectrometer and processed using Agilent Mass Hunter for targeted sucrose quantification. Metabolomic analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Processed metabolomic datasets are openly accessible from the PNNL DataHub project dataset download page and contain secondary processed GC-MS results files and supporting metadata materials linked to relevant source code information supporting data transparency and reuse.

Authors:

Anderson, Lindsey N; Kim, Young-Mo; Waters, Katrina M; Bohutskyi, Pavlo

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); PNNL; Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Publication Date:: Mon Dec 30 04:00:00 UTC 2024

Other Number(s):: PNNL-SA-208061
MSV000096722

DOE Contract Number:: AC05-76RL01830

Research Org.:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

Collaborations:: Predictive Phenomics Initiative, Pacific Northwest National Laboratory

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Carbon Cycling; Circadian Regulation; Metabolomics; Predictive Phenomics; Redox Balancing; S. elongatus PCC 7942; Sucrose Production; gas chromatography-mass spectrometry (GC-MS)

OSTI Identifier:: 2510499

DOI:: https://doi.org/10.25584/2510499

Citation Formats


                    Anderson, Lindsey N, Kim, Young-Mo, Waters, Katrina M, and Bohutskyi, Pavlo. PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5).  United States: N. p., 2024. 
        Web.  doi:10.25584/2510499.

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                    Anderson, Lindsey N, Kim, Young-Mo, Waters, Katrina M, & Bohutskyi, Pavlo. PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5).  United States.  doi:https://doi.org/10.25584/2510499

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                    Anderson, Lindsey N, Kim, Young-Mo, Waters, Katrina M, and Bohutskyi, Pavlo. 2024.  
"PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5)".  United States.  doi:https://doi.org/10.25584/2510499.  https://www.osti.gov/servlets/purl/2510499. Pub date:Mon Dec 30 04:00:00 UTC 2024

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@article{osti_2510499,

  title        = {PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5)},

  author       = {Anderson, Lindsey N and Kim, Young-Mo and Waters, Katrina M and Bohutskyi, Pavlo},

  abstractNote = {The purpose of this experiment was to evaluate how circadian clock regulation impacts carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 in providing insights to strategies for enhanced bioproduction. Culture samples were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours for extracellular sucrose analysis. Circadian metabolomics data was acquired using a Agilent single quadrupole gas chromatography-mass spectrometer and processed using Agilent Mass Hunter for targeted sucrose quantification. Metabolomic analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Processed metabolomic datasets are openly accessible from the PNNL DataHub project dataset download page and contain secondary processed GC-MS results files and supporting metadata materials linked to relevant source code information supporting data transparency and reuse.},

  doi          = {10.25584/2510499},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Dec 30 04:00:00 UTC 2024},

  month        = {Mon Dec 30 04:00:00 UTC 2024}

}

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DOI: https://doi.org/10.25584/2510499

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