S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2)

doi:10.25584/PPI/2475747

Title: S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2) (in EN)

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Abstract

The purpose of this experiment was to examine the redox proteome of S. elongatus PCC 7942 CscB/SPS under different light conditions (light and dark) and culture conditions (dense and dilute optical density). Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify protein cysteines which were differentially oxidized under four conditions. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.

Publication Date:: Sun Oct 20 20:00:00 EDT 2024

Other Number(s):: PNNL-SA-204422
34080

DOE Contract Number:: AC05-76RL01830

Research Org.:: Pacific Northwest National Laboratory 2; PNNL

Sponsoring Org.:: SC

Collaborations:: Predictive Phenomics Initiative, Pacific Northwest National Laboratory

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Predictive Phenomics, S. elongatus PCC 7942, Proteomics, Tandem Mass Tag 16-Plex, global analysis, post translational modifications, central carbon metabolism, circadian rhythm

OSTI Identifier:: 2475747

DOI:: https://doi.org/10.25584/PPI/2475747

Citation Formats


                    S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2).  United States: N. p., 2024. 
        Web.  doi:10.25584/PPI/2475747.

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                    S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2).  United States.  doi:https://doi.org/10.25584/PPI/2475747

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                    2024.  
"S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2)".  United States.  doi:https://doi.org/10.25584/PPI/2475747.  https://www.osti.gov/servlets/purl/2475747. Pub date:Sun Oct 20 20:00:00 EDT 2024

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@article{osti_2475747,

  title        = {S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2)},

  
  abstractNote = {The purpose of this experiment was to examine the redox proteome of S. elongatus PCC 7942 CscB/SPS under different light conditions (light and dark) and culture conditions (dense and dilute optical density). Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify protein cysteines which were differentially oxidized under four conditions. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.},

  doi          = {10.25584/PPI/2475747},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Oct 20 20:00:00 EDT 2024},

  month        = {Sun Oct 20 20:00:00 EDT 2024}

}

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DOI: https://doi.org/10.25584/PPI/2475747

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