U.S. Department of EnergyOffice of Scientific and Technical Information
Microbial Co-Culture Control Proteomics (MC-DP1)
Abstract
Co-cultured S. elongatus PCC 7942 CscB/SPS and R. toruloides IFO0880 presented as an effective photosynthesis-driven biofuel production platform. The goal of this experiment was to understand the molecular mechanism of this co-culture system at redox post-translational modification level. The redox proteome of the co-cultured strains was compared to a mono-cultured S. elongatus in light or dark conditions. Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify modified cysteines at proteome level. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.
- Publication Date:
- Other Number(s):
- PNNL-SA-219351
- DOE Contract Number:
- AC05-76RL01830
- Research Org.:
- PNNL (PNNL2)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- Collaborations:
- Predictive Phenomics Initiative, Pacific Northwest National Laboratory
- OSTI Identifier:
- 3013017
- DOI:
- https://doi.org/10.25584/3013017
Citation Formats
Microbial Co-Culture Control Proteomics (MC-DP1). United States: N. p., 2026.
Web. doi:10.25584/3013017.
Microbial Co-Culture Control Proteomics (MC-DP1). United States. doi:https://doi.org/10.25584/3013017
2026.
"Microbial Co-Culture Control Proteomics (MC-DP1)". United States. doi:https://doi.org/10.25584/3013017. https://www.osti.gov/servlets/purl/3013017. Pub date:Wed Jan 07 19:00:00 EST 2026
@article{osti_3013017,
title = {Microbial Co-Culture Control Proteomics (MC-DP1)},
abstractNote = {Co-cultured S. elongatus PCC 7942 CscB/SPS and R. toruloides IFO0880 presented as an effective photosynthesis-driven biofuel production platform. The goal of this experiment was to understand the molecular mechanism of this co-culture system at redox post-translational modification level. The redox proteome of the co-cultured strains was compared to a mono-cultured S. elongatus in light or dark conditions. Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify modified cysteines at proteome level. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.},
doi = {10.25584/3013017},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 07 19:00:00 EST 2026},
month = {Wed Jan 07 19:00:00 EST 2026}
}
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