PPI DataHub Project Data Package: High-density Lipoprotein (HDL) Structure and Function Proteomics

Sarkar, Snigdha; Morris, Jamie; You, Youngki; Street, Scott E.; Thibert, Stephanie; Attah, Isaac K.; Hutchinson-Bunch, Chelsea; Novikova, Irina; Evans, James; Vaisar, Tomas; Davidson, William S.; Melchior, John T.

doi:10.25584/PPI/2447854

Title: PPI DataHub Project Data Package: High-density Lipoprotein (HDL) Structure and Function Proteomics

Dataset
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Abstract

The purpose of this experiment was to investigate how the interactions between APOA1 and APOA2 on the surface of high-density lipoproteins (HDL) impact particle function. Interactions were investigated on HDL isolated from human blood plasma using structural proteomics tools such as chemical cross-linking and limited proteolysis (LiP). The structural proteomics data was acquired using a Q-Exactive HF-X mass spectrometer and data was processed and compiled using MaxQuant sofware (v.1.6.17.0). Processed datasets are openly accessible from the download button (~2.8 GB) and contain secondary processed LiP and global proteomic results files and supporting metadata materials. Processed data downloads include a sample naming key, processed MaxQuant results/parameters, and protein annotated relative abundance files.

Authors:

; Morris, Jamie;

; Street, Scott E.; Thibert, Stephanie;

;

Pacific Northwest National Laboratory; PNNL
Department of Pathology and Laboratory Medicine, University of Cincinnati
Pacific Northwest National Laboratory
Department of Medicine, University of Washington School of Medicine

Publication Date:: Tue Sep 24 04:00:00 UTC 2024

Other Number(s):: 5837; 34063

DOE Contract Number:: AC05-76RL01830

Research Org.:: Pacific Northwest National Laboratory 2; PNNL

Sponsoring Org.:: SC-BER

Collaborations:: Predictive Phenomics Initiative, Pacific Northwest National Laboratory; Department of Pathology and Laboratory Medicine, University of Cincinnati; Department of Medicine, University of Washington School of Medicine

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Apolipoprotein A-I; Apolipoprotein A-II ABCA1; Apolipoproteins; High Density Lipoproteins; Limited Proteolysis Proteomics; Lipoprotein metabolism; Lipoproteins Structure; cholesterol efflux

OSTI Identifier:: 2447854

DOI:: https://doi.org/10.25584/PPI/2447854

Citation Formats


                    Sarkar, Snigdha, Morris, Jamie, You, Youngki, Street, Scott E., Thibert, Stephanie, Attah, Isaac K., Hutchinson-Bunch, Chelsea, Novikova, Irina, Evans, James, Vaisar, Tomas, Davidson, William S., and Melchior, John T. PPI DataHub Project Data Package: High-density Lipoprotein (HDL) Structure and Function Proteomics.  United States: N. p., 2024. 
        Web.  doi:10.25584/PPI/2447854.

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                    Sarkar, Snigdha, Morris, Jamie, You, Youngki, Street, Scott E., Thibert, Stephanie, Attah, Isaac K., Hutchinson-Bunch, Chelsea, Novikova, Irina, Evans, James, Vaisar, Tomas, Davidson, William S., & Melchior, John T. PPI DataHub Project Data Package: High-density Lipoprotein (HDL) Structure and Function Proteomics.  United States.  doi:https://doi.org/10.25584/PPI/2447854

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                    Sarkar, Snigdha, Morris, Jamie, You, Youngki, Street, Scott E., Thibert, Stephanie, Attah, Isaac K., Hutchinson-Bunch, Chelsea, Novikova, Irina, Evans, James, Vaisar, Tomas, Davidson, William S., and Melchior, John T. 2024.  
"PPI DataHub Project Data Package: High-density Lipoprotein (HDL) Structure and Function Proteomics".  United States.  doi:https://doi.org/10.25584/PPI/2447854.  https://www.osti.gov/servlets/purl/2447854. Pub date:Tue Sep 24 04:00:00 UTC 2024

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@article{osti_2447854,

  title        = {PPI DataHub Project Data Package: High-density Lipoprotein (HDL) Structure and Function Proteomics},

  author       = {Sarkar, Snigdha and Morris, Jamie and You, Youngki and Street, Scott E. and Thibert, Stephanie and Attah, Isaac K. and Hutchinson-Bunch, Chelsea and Novikova, Irina and Evans, James and Vaisar, Tomas and Davidson, William S. and Melchior, John T.},

  abstractNote = {The purpose of this experiment was to investigate how the interactions between APOA1 and APOA2 on the surface of high-density lipoproteins (HDL) impact particle function. Interactions were investigated on HDL isolated from human blood plasma using structural proteomics tools such as chemical cross-linking and limited proteolysis (LiP). The structural proteomics data was acquired using a Q-Exactive HF-X mass spectrometer and data was processed and compiled using MaxQuant sofware (v.1.6.17.0). Processed datasets are openly accessible from the download button (~2.8 GB) and contain secondary processed LiP and global proteomic results files and supporting metadata materials. Processed data downloads include a sample naming key, processed MaxQuant results/parameters, and protein annotated relative abundance files.},

  doi          = {10.25584/PPI/2447854},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Sep 24 04:00:00 UTC 2024},

  month        = {Tue Sep 24 04:00:00 UTC 2024}

}

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DOI: https://doi.org/10.25584/PPI/2447854

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