U.S. Department of EnergyOffice of Scientific and Technical Information
Human Liver Epithelium Response to HCoV-229E Infection Epigenomics (ACS-DP4)
Abstract
The purpose of this experiment was to evaluate how wild-type Human coronavirus strain 229E (HCoV-229E) infection alters chromatin accessibility in infected cells only. Sample data was obtained for mock and infected (standard and UV-inactivated) immortalized human liver cells (HuH-7) and collected 24 hrs. post infection. Samples were processed using assay for transposase-accessible chromatin using high-throughput sequencing (ATAC-Seq) and generated bar coded library samples were evaluated for RNA sequencing (RNA-Seq) expression analysis. Processed ATAC-Seq datasets are openly accessible from the download button and contain secondary processed RNA-Seq results files and supporting metadata materials. Data download includes a sample naming key, infection titer metadata, normalized counts, and relevant computational source code information supporting data transparency and reuse.
- Authors:
-
;
;
;
- Pacific Northwest National Laboratory (PNNL); PNNL
- Pacific Northwest National Laboratory (PNNL)
- Publication Date:
- Other Number(s):
- PNNL-SA-204870
30487
- DOE Contract Number:
- AC05-76RL01830
- Research Org.:
- Pacific Northwest National Laboratory 2; PNNL
- Sponsoring Org.:
- SC
- Collaborations:
- Predictive Phenomics Initiative, Pacific Northwest National Laboratory
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Predictive Phenomics, ATAC-Seq, Lethal Human Viruses, HCov-229E, Homo sapiens, Epigenomics
- OSTI Identifier:
- 2475746
- DOI:
- https://doi.org/10.25584/PPI/2475746
Citation Formats
Sims, Amy C, Leiser, Owen P, Anderson, Lindsey N, and Waters, Katrina M. Human Liver Epithelium Response to HCoV-229E Infection Epigenomics (ACS-DP4). United States: N. p., 2024.
Web. doi:10.25584/PPI/2475746.
Sims, Amy C, Leiser, Owen P, Anderson, Lindsey N, & Waters, Katrina M. Human Liver Epithelium Response to HCoV-229E Infection Epigenomics (ACS-DP4). United States. doi:https://doi.org/10.25584/PPI/2475746
Sims, Amy C, Leiser, Owen P, Anderson, Lindsey N, and Waters, Katrina M. 2024.
"Human Liver Epithelium Response to HCoV-229E Infection Epigenomics (ACS-DP4)". United States. doi:https://doi.org/10.25584/PPI/2475746. https://www.osti.gov/servlets/purl/2475746. Pub date:Wed Sep 25 00:00:00 EDT 2024
@article{osti_2475746,
title = {Human Liver Epithelium Response to HCoV-229E Infection Epigenomics (ACS-DP4)},
author = {Sims, Amy C and Leiser, Owen P and Anderson, Lindsey N and Waters, Katrina M},
abstractNote = {The purpose of this experiment was to evaluate how wild-type Human coronavirus strain 229E (HCoV-229E) infection alters chromatin accessibility in infected cells only. Sample data was obtained for mock and infected (standard and UV-inactivated) immortalized human liver cells (HuH-7) and collected 24 hrs. post infection. Samples were processed using assay for transposase-accessible chromatin using high-throughput sequencing (ATAC-Seq) and generated bar coded library samples were evaluated for RNA sequencing (RNA-Seq) expression analysis. Processed ATAC-Seq datasets are openly accessible from the download button and contain secondary processed RNA-Seq results files and supporting metadata materials. Data download includes a sample naming key, infection titer metadata, normalized counts, and relevant computational source code information supporting data transparency and reuse.},
doi = {10.25584/PPI/2475746},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Sep 25 00:00:00 EDT 2024},
month = {Wed Sep 25 00:00:00 EDT 2024}
}