The future of liquid chromatography-mass spectrometry in metabolic profiling and metabolomic studies for biomarker discovery.

Journal Article · Fri Jun 01 04:00:00 EDT 2007 · Biomarkers in Medicine, 1(1):159-185

DOI:https://doi.org/10.2217/17520363.1.1.159· OSTI ID:985062

Metz, Thomas O.; Zhang, Qibin; Page, Jason S.; Shen, Yufeng; Callister, Stephen J.; Jacobs, Jon M.; Smith, Richard D.

The future utility of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discover will be discussed, beginning with a brief description of the evolution of metabolomics and the utilization of the three most popular analytical platforms in such studies: NMR, GC-MS, and LC-MS. Emphasis is placed on recent developments in high-efficiency LC separations and sensitive electrospray ionization approaches and the benefits to incorporating both in LC-MS-based approaches. The advantages and disadvantages of various quantitative approaches are reviewed, followed by the current LC-MS-based tools available for candidate biomarker characterization and identification. Finally, a brief prediction on the future path of LC-MS-based methods in metabolic profiling and metabolomic studies is given.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 985062
• Report Number(s): PNNL-SA-53806; 16303; 12500a; 16720; 400412000
• Journal Information: Biomarkers in Medicine, 1(1):159-185, Journal Name: Biomarkers in Medicine, 1(1):159-185 Journal Issue: 1 Vol. 1; ISSN 1752-0363; ISSN 1752-0371
• Country of Publication: United States
• Language: English

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Environmental Molecular Sciences Laboratory