High Resolution Separations and Improved Ion Production and Transmission in Metabolomics

Journal Article · Mon Mar 31 04:00:00 EDT 2008 · Trends in Analytical Chemistry. TrAC, 27(3):205-214

DOI:https://doi.org/10.1016/j.trac.2007.11.003· OSTI ID:927706

Metz, Thomas O; Page, Jason S; Baker, Erin Shammel; Tang, Keqi; Ding, Jie; Shen, Yufeng; Smith, Richard D

The goal of metabolomics experiments is the detection and quantitation of as many sample components as reasonably possible in order to identify “features” that can be used to characterize the samples under study. When utilizing electrospray ionization to produce ions for analysis by mass spectrometry (MS), it is imperative that metabolome sample constituents be efficiently separated prior to ion production, in order to minimize the phenomenon of ionization suppression. Similarly, optimization of the MS inlet can lead to increased measurement sensitivity. This review will focus on the role of high resolution liquid chromatography (LC) separations in conjunction with improved ion production and transmission for LC-MS-based metabolomics.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 927706
• Report Number(s): PNNL-SA-56841; 12500a; 24698; 400412000
• Journal Information: Trends in Analytical Chemistry. TrAC, 27(3):205-214, Journal Name: Trends in Analytical Chemistry. TrAC, 27(3):205-214 Journal Issue: 3 Vol. 27
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Environmental Molecular Sciences Laboratory
IONIZATION
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MULTI-ELEMENT ANALYSIS
RESOLUTION
SAMPLE PREPARATION
SENSITIVITY