Conventional and Advanced Separations in Mass Spectrometry-Based Metabolomics: Methodologies and Applications

Book · Mon Feb 15 23:00:00 EST 2016

DOI:https://doi.org/10.1016/B978-0-12-409547-2.12132-8· OSTI ID:1327151

Heyman, Heino M.; Zhang, Xing; Tang, Keqi; Baker, Erin Shammel; Metz, Thomas O.

Metabolomics is the quantitative analysis of all metabolites in a given sample. Due to the chemical complexity of the metabolome, optimal separations are required for comprehensive identification and quantification of sample constituents. This chapter provides an overview of both conventional and advanced separations methods in practice for reducing the complexity of metabolite extracts delivered to the mass spectrometer detector, and covers gas chromatography (GC), liquid chromatography (LC), capillary electrophoresis (CE), supercritical fluid chromatography (SFC) and ion mobility spectrometry (IMS) separation techniques coupled with mass spectrometry (MS) as both uni-dimensional and as multi-dimensional approaches.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1327151
• Report Number(s): PNNL-SA-111253; 48679; KP1601010
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory
GCxGC – MS
LCxLC – MS
capillary electrophoresis – mass spectrometry (CE-MS)
coupled separation
gas chromatography – mass spectrometry (GC-MS)
ion mobility spectrometry (IMS)
liquid chromatography – mass spectrometry (LC-MS)
metabolomics
multi-dimensional chromatography
supercritical fluid chromatography – mass spectrometry (SFC – MS)