High temperature flow-through device for rapid solubilization and analysis

West, Jason A. A.; Hukari, Kyle W; Patel, Kamlesh D; Peterson, Kenneth A; Renzi, Ronald F

High temperature flow-through device for rapid solubilization and analysis

Patent · Tue Sep 22 04:00:00 EDT 2009

OSTI ID:971614

West, Jason A. A. ^[1]; Hukari, Kyle W ^[2]; Patel, Kamlesh D ^[3]; Peterson, Kenneth A ^[4]; Renzi, Ronald F ^[5]

Castro Valley, CA
San Ramon, CA
Dublin, CA
Albuquerque, NM
Tracy, CA

Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

View Patent

Research Organization:: Sandia National Laboratories (SNL-CA), Livermore, CA

Sponsoring Organization:: United States Department of Energy

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia National Laboratories (Liverpool, CA)

Patent Number(s):: 7,592,139

Application Number:: 11/059,079

OSTI ID:: 971614

Country of Publication:: United States

Language:: English

References (43)

Measurement of alkaline phosphatase isoenzymes in individual mouse bone marrow fibroblast cells based on capillary electrophoresis with on-capillary enzyme-catalyzed reaction and electrochemical detection Sun, Xuemei; Jin, Wenrui; Li, Dong ELECTROPHORESIS, Vol. 25, Issue 12 https://doi.org/10.1002/elps.200305813	journal	June 2004
Continuous Cell Introduction for the Analysis of Individual Cells by Capillary Electrophoresis Chen, Shujun; Lillard, Sheri J. Analytical Chemistry, Vol. 73, Issue 1 https://doi.org/10.1021/ac0009088	journal	January 2001
A One-Step Procedure for Isolation of Poly(A) ⁺ mRNA from Isolated Brain Capillaries and Endothelial Cells in Culture Boado, Ruben J.; Pardridge, William M. Journal of Neurochemistry, Vol. 57, Issue 6 https://doi.org/10.1111/j.1471-4159.1991.tb06433.x	journal	December 1991
Capillary Electrophoresis of Microbes Moon, Byoung-Geoun; Lee, Yong-Ill; Kang, Seong-Ho Bulletin of the Korean Chemical Society, Vol. 24, Issue 1, p. 81-85 https://doi.org/10.5012/bkcs.2003.24.1.081	journal	January 2003
A rapid and simple method for the isolation of apoptotic DNA fragments Herrmann, M.; Lorenz, H. -M.; Voll, R. Nucleic Acids Research, Vol. 22, Issue 24 https://doi.org/10.1093/nar/22.24.5506	journal	January 1994
Capillary-based fully integrated and automated system for nanoliter polymerase chain reaction analysis directly from cheek cells He, Yan; Zhang, Yonghua H.; Yeung, Edward S. Journal of Chromatography A, Vol. 924, Issue 1-2 https://doi.org/10.1016/S0021-9673(01)00828-7	journal	July 2001
Quantitative Detection of Hepatitis B Virus DNA by Real-Time Nucleic Acid Sequence-Based Amplification with Molecular Beacon Detection Yates, S.; Penning, M.; Goudsmit, J. Journal of Clinical Microbiology, Vol. 39, Issue 10 https://doi.org/10.1128/JCM.39.10.3656-3665.2001	journal	October 2001
Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method Malorny, B. International Journal of Food Microbiology, Vol. 89, Issue 2-3 https://doi.org/10.1016/S0168-1605(03)00154-5	journal	December 2003
Subcellular metabolite profiles of the parent CCRF-CEM and the derived CEM/C2 cell lines after treatment with doxorubicin Anderson, A. Journal of Chromatography B, Vol. 808, Issue 2 https://doi.org/10.1016/j.jchromb.2004.05.017	journal	September 2004
Multiplexed Automated DNA Sequencing Directly from Single Bacterial Colonies Zhang, Yonghua; Tan, Hongdong; Yeung, Edward S. Analytical Chemistry, Vol. 71, Issue 22 https://doi.org/10.1021/ac9904462	journal	November 1999
Instrumentation for Chemical Cytometry Krylov, Sergey N.; Starke, Dieter A.; Arriaga, Edgar A. Analytical Chemistry, Vol. 72, Issue 4 https://doi.org/10.1021/ac991096m	journal	February 2000
New Method for the Extraction of DNA from White Blood Cells for the Detection of Common Genetic Variants Associated with Thrombophilia Angelini, Antonio; Di Febbo, Concetta; Rullo, Agnese Pathophysiology of Haemostasis and Thrombosis, Vol. 32, Issue 4 https://doi.org/10.1159/000070424	journal	January 2002
Monolithic Porous Polymer for On-Chip Solid-Phase Extraction and Preconcentration Prepared by Photoinitiated in Situ Polymerization within a Microfluidic Device Yu, Cong; Davey, Mark H.; Svec, Frantisek Analytical Chemistry, Vol. 73, Issue 21 https://doi.org/10.1021/ac0106288	journal	November 2001
Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip Cheek, Brady J.; Steel, Adam B.; Torres, Matthew P. Analytical Chemistry, Vol. 73, Issue 24 https://doi.org/10.1021/ac0108616	journal	December 2001
Towards stationary phases for chromatography on a microchip: Molded porous polymer monoliths prepared in capillaries by photoinitiated in situ polymerization as separation media for electrochromatography Yu, Cong; Svec, Frantisek; Fréchet, Jean M. J. Electrophoresis, Vol. 21, Issue 1, p. 120-127 https://doi.org/10.1002/(SICI)1522-2683(20000101)21:1<120::AID-ELPS120>3.0.CO;2-H	journal	January 2000
Quantification of the carbazole 1,9a-dioxygenase gene by real-time competitive PCR combined with co-extraction of internal standards Widada, Jaka; Nojiri, Hideaki; Kasuga, Kano FEMS Microbiology Letters, Vol. 202, Issue 1 https://doi.org/10.1111/j.1574-6968.2001.tb10779.x	journal	August 2001
Bioethics and Bioterrorism Garland, Brent The Journal of Philosophy, Science & Law, Vol. 2, Issue 3 https://doi.org/10.5840/jpsl2002233	journal	January 2002
Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chipElectronic supplementary information (ESI) available: Video showing a single erythrocyte transported through the microchannel, docking (adhering) at a particular point and then being lysed. See http://www.rsc.org/suppdata/lc/b3/b310552k/ Gao, Jian; Yin, Xue-Feng; Fang, Zhao-Lun Lab on a Chip, Vol. 4, Issue 1 https://doi.org/10.1039/b310552k	journal	January 2004
HPCE Monitoring of the N-Glycosylation Pattern and Sialylation of Murine Erythropoietin Produced by CHO Cells in Batch Processes LeFloch, F.; Tessier, B.; Chenuet, S. Biotechnology Progress, Vol. 20, Issue 3 https://doi.org/10.1021/bp0343211	journal	June 2004
Protein profile characterization of bacterial lysates by capillary electrophoresis Kustos, Ildikó; Kocsis, Béla; Kerepesi, Ildikó Electrophoresis, Vol. 19, Issue 13 https://doi.org/10.1002/elps.1150191311	journal	October 1998
Self-Assembling Protein Microarrays Ramachandran, N. Science, Vol. 305, Issue 5680 https://doi.org/10.1126/science.1097639	journal	July 2004
Evaluation of DNA Fragment Sizing and Quantification by the Agilent 2100 Bioanalyzer Panaro, Nicholas J.; Yuen, Po K. i.; Sakazume, Taku Clinical Chemistry, Vol. 46, Issue 11 https://doi.org/10.1093/clinchem/46.11.1851	journal	November 2000
Laser−Micropipet Combination for Single-Cell Analysis Sims, Christopher E.; Meredith, Gavin D.; Krasieva, Tatiana B. Analytical Chemistry, Vol. 70, Issue 21 https://doi.org/10.1021/ac9802269	journal	November 1998
Microfluidic Devices for the High-Throughput Chemical Analysis of Cells McClain, Maxine A.; Culbertson, Christopher T.; Jacobson, Stephen C. Analytical Chemistry, Vol. 75, Issue 21 https://doi.org/10.1021/ac0346510	journal	November 2003
Capillary Isoelectric Focusing−Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Protein Characterization Yang, Liyu; Lee, Cheng S.; Hofstadler, Steven A. Analytical Chemistry, Vol. 70, Issue 15 https://doi.org/10.1021/ac980224o	journal	August 1998
Antiproliferative Effects of △²⁴⁽²⁵⁾ Sterol Methyl Transferase Inhibitors on Trypanosoma (Schizotrypanum) cruzi: In vitro and in vivo Studies Urbina, Julio A.; Vivas, Julio; Lazardi, Keyla Chemotherapy, Vol. 42, Issue 4 https://doi.org/10.1159/000239458	journal	January 1996
μChemLab: an integrated microanalytical system for chemical analysis using parallel gas and liquid phase microseparations Thomas, Gregory A.; Frye-Mason, Gregory C.; Bailey, Christopher A. AeroSense '99, SPIE Proceedings https://doi.org/10.1117/12.357145	conference	July 1999
Highly protective alkalinization by ammonia vapor diffusion in viscosimetric DNA damage assessment Zahn, Rudolf K.; Zahn-Daimler, Gertrud; Beyer, Rudolf Analytical Biochemistry, Vol. 168, Issue 2 https://doi.org/10.1016/0003-2697(88)90334-X	journal	February 1988
Fermentation, Purification, and Characterization of Protective Antigen from a Recombinant, Avirulent Strain ofBacillus anthracis Farchaus, J. W.; Ribot, W. J.; Jendrek, S. Applied and Environmental Microbiology, Vol. 64, Issue 3 https://doi.org/10.1128/AEM.64.3.982-991.1998	journal	March 1998
Multichannel arrays on polymer substrates: toward a disposable proteomics chip Becker, Holger; Ehrfeld, Wolfgang; Pommersheim, Rainer Design, Test, and Microfabrication of MEMS/MOEMS, SPIE Proceedings https://doi.org/10.1117/12.341266	conference	March 1999
Electroosmotically Induced Hydraulic Pumping on Microchips Alarie, Jean Pierre; Jacobson, Stephen C.; Broyles, B. Scott Micro Total Analysis Systems 2001 https://doi.org/10.1007/978-94-010-1015-3_53	book	January 2001
Detection of doxorubicin and metabolites in cell extracts and in single cells by capillary electrophoresis with laser-induced fluorescence detection Anderson, Adrian B.; Gergen, Jamie; Arriaga, Edgar A. Journal of Chromatography B, Vol. 769, Issue 1 https://doi.org/10.1016/S1570-0232(01)00633-X	journal	March 2002
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane) Duffy, David C.; McDonald, J. Cooper; Schueller, Olivier J. A. Analytical Chemistry, Vol. 70, Issue 23, p. 4974-4984 https://doi.org/10.1021/ac980656z	journal	October 1998
Mycobacterial proteome extraction: Comparison of disruption methods Lanigan, Mark D.; Vaughan, Jill A.; Shiell, Brian J. PROTEOMICS, Vol. 4, Issue 4 https://doi.org/10.1002/pmic.200300672	journal	April 2004
Hand-Held Microanalytical Instrument for Chip-Based Electrophoretic Separations of Proteins Renzi, Ronald F.; Stamps, James; Horn, Brent A. Analytical Chemistry, Vol. 77, Issue 2 https://doi.org/10.1021/ac049214f	journal	January 2005
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray Schena, M.; Shalon, D.; Davis, R. W. Science, Vol. 270, Issue 5235 https://doi.org/10.1126/science.270.5235.467	journal	October 1995
Measurement of Single-Cell Gene Expression Using Capillary Electrophoresis Zabzdyr, Jennifer L.; Lillard, Sheri J. Analytical Chemistry, Vol. 73, Issue 23 https://doi.org/10.1021/ac0155714	journal	December 2001
High-throughput screening of soluble recombinant proteins Shih, Yan-Ping; Kung, Wen-Mei; Chen, Jui-Chuan Protein Science, Vol. 11, Issue 7 https://doi.org/10.1110/ps.0205202	journal	July 2002
Determination of endogenous extracellular signal-regulated protein kinase by microchip capillary electrophoresis Starkey, Dustin E.; Abdelaziez, Yasser; Ahn, Chong H. Analytical Biochemistry, Vol. 316, Issue 2 https://doi.org/10.1016/S0003-2697(03)00058-7	journal	May 2003
Fast Electrical Lysis of Cells for Capillary Electrophoresis Han, Futian; Wang, Yan; Sims, Christopher E. Analytical Chemistry, Vol. 75, Issue 15 https://doi.org/10.1021/ac0341970	journal	August 2003
Isolation of micronuclei from Euplotes octocarinatus and identification of an internal eliminated sequence in the micronuclear gene encoding γ-tubulin 2 Tan, Ming; Brünen-Nieweler, Claudia; Heckmann, Klaus European Journal of Protistology, Vol. 35, Issue 2 https://doi.org/10.1016/S0932-4739(99)80039-X	journal	June 1999
Development of a Single-Tube, Cell Lysis-Based, Genus-Specific PCR Method for Rapid Identification of Mycobacteria: Optimization of Cell Lysis, PCR Primers and Conditions, and Restriction Pattern Analysis Khan, I. U. H.; Yadav, J. S. Journal of Clinical Microbiology, Vol. 42, Issue 1 https://doi.org/10.1128/JCM.42.1.453-457.2004	journal	January 2004
Genetic Control of Capsid Length in Bacteriophage T4 I. Isolation and Preliminary Description of Four New Mutants Doermann, A. H.; Eiserling, F. A.; Boehner, Linde Journal of Virology, Vol. 12, Issue 2 https://doi.org/10.1128/JVI.12.2.374-385.1973	journal	January 1973

Similar Records

High temperature flow-through device for rapid solubilization and analysis

Patent · Tue Apr 23 00:00:00 EDT 2013 · OSTI ID:1083601

Universal nucleic acids sample preparation method for cells, spores and their mixture

High temperature flow-through device for rapid solubilization and analysis

Citation Formats

References (43)

Similar Records

Related Subjects