Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Protein detection system

Patent ·
OSTI ID:963807
 [1];  [2];  [3];  [1]
  1. Livermore, CA
  2. Bethesda, MD
  3. San Ramon, CA
+ Show Author Affiliations
The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.
Research Organization:
Sandia National Laboratories (SNL-CA), Livermore, CA
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
AC04-94AL85000
Assignee:
Sandia Corporation (Livermore, CA)
Patent Number(s):
7,527,977
Application Number:
11/087,942
OSTI ID:
963807
Country of Publication:
United States
Language:
English

References (5)

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy journal April 2002
Gene probe assays on a fibre-optic evanescent wave biosensor journal January 1992
Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity journal August 1997
Cavity ring down spectroscopy on solid C60 journal February 1999
Cavity ring-down spectroscopy in the liquid phase journal February 2002

Similar Records

Development of {micro}Chemlab{sup TM}/CG for Detection of Biotoxins
Conference · Mon Oct 23 00:00:00 EDT 2000 · OSTI ID:772349
Continuous-Flow Detector for Rapid Pathogen Identification
Technical Report · Fri Sep 01 00:00:00 EDT 2006 · OSTI ID:1323909
Bioaerosol collection and concentration for microseparations-based detectors.
Technical Report · Mon Feb 28 23:00:00 EST 2005 · OSTI ID:878588

Related Subjects

47 OTHER INSTRUMENTATION