Development of {micro}Chemlab{sup TM}/CG for Detection of Biotoxins
Conference
·
OSTI ID:772349
The authors have developed a research prototype device for low level detection of SEB, ricin, botulinum toxin and ovalbumin in liquid samples. This hand-held, low power device analyzes samples by capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE) in a parallel microchannel format, and provides sensitive detection of fluorescently-tagged analytes using miniature 392 nm diode laser-induced fluorescence. They have successfully demonstrated simultaneous parallel channel separations, fully automated high voltage control of fluidics and automated data analysis with toxin identification and semi-quantitation in the presence of aerosol backgrounds in a stand-alone self-contained unit. Tests of the fully integrated device indicate high sensitivity detection (as low as 5 nM) and cycle times of 5-10 minutes.
- Research Organization:
- Sandia National Labs., Albuquerque, NM (US); Sandia National Labs., Livermore, CA (US)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- AC04-94AL85000
- OSTI ID:
- 772349
- Report Number(s):
- SAND2001-8107C
- Country of Publication:
- United States
- Language:
- English
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