Protein detection system

Patent · Tue May 05 00:00:00 EDT 2009

OSTI ID:963807

Fruetel, Julie A ^[1]; Fiechtner, Gregory J ^[2]; Kliner, Dahv A. V. ^[3]; McIlroy, Andrew ^[1]

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1. Livermore, CA
2. Bethesda, MD
3. San Ramon, CA

The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.

• Research Organization: Sandia National Lab. (SNL-CA), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC04-94AL85000
• Assignee: Sandia Corporation (Livermore, CA)
• Patent Number(s): 7,527,977
• Application Number: 11/087,942
• OSTI ID: 963807
• Country of Publication: United States
• Language: English

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