Impact of phenolic substrate and growth temperature on the arthrobacter chlorophenolicus proteome
Journal Article
·
· Journal of Proteome Research
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol or phenol at 5 C and 28 C; both for the wild type and a mutant strain with mass spectrometry based proteomics. A label free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- Earth Sciences Division
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 949047
- Report Number(s):
- LBNL-1565E
- Journal Information:
- Journal of Proteome Research, Journal Name: Journal of Proteome Research
- Country of Publication:
- United States
- Language:
- English
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