Global proteomic analysis of the chromate response in Arthrobacter sp strain FB24.
A global proteomic evaluation of the response of Arthrobacter sp. strain FB24 to 5 and 20 mM Cr(VI) was conducted using both two-dimensional gel electrophoresis (2-DGE) and liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS). The changes in protein expression found with 2-DGE indicate alterations in central metabolism and amino acid synthesis. Proteome coverage increased from 22% with 2-DGE to 71% with LC/LC-MS/MS. The proteins exhibiting the highest levels of expression under Cr(VI) stress suggest intracellular sulfur limitation, which could be driven by competition for the sulfate (SO{sub 4}{sup 2-}) transporter by the chromate (CrO{sub 4}{sup 2-}) ion. These results are consistent with the growth defects seen with strain FB24 when Cr(VI) concentrations exceeded 5 mM.
- Research Organization:
- Argonne National Laboratory (ANL)
- Sponsoring Organization:
- SC; Purdue Research Foundation; Bilsland Doctoral Fellowship
- DOE Contract Number:
- AC02-06CH11357
- OSTI ID:
- 990164
- Report Number(s):
- ANL/BIO/JA-62588
- Journal Information:
- J. Proteome Res., Journal Name: J. Proteome Res. Journal Issue: 4 ; Apr. 2009 Vol. 8; ISSN 1535-3893
- Country of Publication:
- United States
- Language:
- ENGLISH
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