Global Proteomic Analysis of the Chromate Response in Arthrobacter sp strain FB24
A global proteomic evaluation of the response of Arthrobacter sp. strain FB24 to 5 mM and 20 mM Cr(VI) was conducted using both two-dimensional gel electrophoresis (2-DGE) and liquid chromatography coupled to tandem mass spectrometry (LC/LC-MS/MS). The changes in protein expression found with 2-DGE indicate alterations in central metabolism and amino acid synthesis. Proteome coverage increased from 22% with 2-DGE to 71% with LC/LC-MS/MS. The proteins exhibiting the highest levels of expression under Cr(VI) stress suggest intracellular sulfur limitation, which could be driven by competition for the sulfate (SO42-) transporter by the chromate (CrO42-) ion. These results are consistent with the growth defects seen with strain FB24 when Cr(VI) concentrations exceed 5 mM.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 974963
- Report Number(s):
- PNNL-SA-62660
- Journal Information:
- Journal of Proteome Research, 8(4)(Sp.Iss.SI):1704-16, Journal Name: Journal of Proteome Research, 8(4)(Sp.Iss.SI):1704-16 Journal Issue: 4 Vol. 8
- Country of Publication:
- United States
- Language:
- English
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