Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome

Journal Article · Thu Jan 01 04:00:00 EST 2009 · Journal of Proteome Research

DOI:https://doi.org/10.1021/pr800897c· OSTI ID:1081786

Unell, Maria ^[1]; Abraham, Paul E ^[2]; Shah, Manesh B ^[2]; Zhang, B ^[3]; Ruckert, Christian ^[4]; Verberkmoes, Nathan C ^[2]; Jansson, J ^[1]

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1. Swedish University of Agricultural Sciences, Upsalla, Sweden
2. ORNL
3. Vanderbilt University
4. Bielefeld University, Center for Biotechnology, Bielefeld, Germany

We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol- degradation pathway was confirmed, but not that for phenol.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1081786
• Journal Information: Journal of Proteome Research, Journal Name: Journal of Proteome Research Journal Issue: 4 Vol. 8; ISSN 1535-3893
• Country of Publication: United States
• Language: English

Related Subjects

4-chlorophenol
4-nitrophenol
Arthrobacter chlorophenolicus
phenol
shotgun proteomics