Chromosomal mosaicism in mouse two-cell embryos after paternal exposure to acrylamide
Chromosomal mosaicism in human preimplantation embryos is a common cause ofspontaneous abortions, however, our knowledge of its etiology is limited. We used multicolor fluorescence in situ hybridization (FISH) painting to investigate whether paternally-transmitted chromosomal aberrations result in mosaicism in mouse 2-cell embryos. Paternal exposure to acrylamide, an important industrial chemical also found in tobacco smoke and generated during the cooking process of starchy foods, produced significant increases in chromosomally defective 2-cell embryos, however, the effects were transient primarily affecting the postmeiotic stages of spermatogenesis. Comparisons with our previous study of zygotes demonstrated similar frequencies of chromosomally abnormal zygotes and 2-cell embryos suggesting that there was no apparent selection against numerical or structural chromosomal aberrations. However, the majority of affected 2-cell embryos were mosaics showing different chromosomal abnormalities in the two blastomeric metaphases. Analyses of chromosomal aberrations in zygotes and 2-cell embryos showed a tendency for loss of acentric fragments during the first mitotic division ofembryogenesis, while both dicentrics and translocations apparently underwent propersegregation. These results suggest that embryonic development can proceed up to the end of the second cell cycle of development in the presence of abnormal paternal chromosomes and that even dicentrics can persist through cell division. The high incidence of chromosomally mosaic 2-cell embryos suggests that the first mitotic division of embryogenesis is prone to missegregation errors and that paternally-transmitted chromosomal abnromalities increase the risk of missegregation leading to embryonic mosaicism.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- Life Sciences Division
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 946593
- Report Number(s):
- LBNL-1392E
- Journal Information:
- Toxicological Sciences, Journal Name: Toxicological Sciences Journal Issue: 1 Vol. 107; ISSN 1096-6080
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACRYLAMIDE
CELL CYCLE
CELL DIVISION
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CULTURAL OBJECTS
EMBRYOS
ETIOLOGY
FLUORESCENCE
IN-SITU HYBRIDIZATION
MOSAICISM
ONTOGENESIS
SEGREGATION
SPERMATOGENESIS
TOBACCO SMOKES
TRANSIENTS
ZYGOTES
acrylamide
in vivo
fluorescence in situ hybridization