Crystallization and Characterization of the Thallium Form of the Oxytricha Nova G-Quadruplex

Journal Article · Sun Jan 01 04:00:00 EST 2006 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkl616· OSTI ID:930204

Gill, M; Strobel, S; Loria, J

The crystal structure of the Tl{sup +} form of the G-quadruplex formed from the Oxytricha nova telomere sequence, d(G{sub 4}T{sub 4}G{sub 4}), has been solved to 1.55 {angstrom}. This G-quadruplex contains five Tl{sup +} ions, three of which are interspersed between adjacent G-quartet planes and one in each of the two thymine loops. The structure displays a high degree of similarity to the K{sup +} crystal structure [Haider et al. (2002), J. Mol. Biol., 320, 189-200], including the number and location of the monovalent cation binding sites. The highly isomorphic nature of the two structures, which contain such a large number of monovalent binding sites (relative to nucleic acid content), verifies the ability of Tl{sup +} to mimic K{sup +} in nucleic acids. Information from this report confirms and extends the assignment of {sup 205}Tl resonances from a previous report [Gill et al. (2005), J. Am. Chem. Soc., 127, 16 723-16 732] where {sup 205}Tl NMR was used to study monovalent cation binding to this G-quadruplex. The assignment of these resonances provides evidence for the occurrence of conformational dynamics in the thymine loop region that is in slow exchange on the {sup 205}Tl timescale.

• Research Organization: Brookhaven National Laboratory (BNL) National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 930204
• Report Number(s): BNL--80867-2008-JA
• Journal Information: Nucleic Acids Research, Journal Name: Nucleic Acids Research Vol. 34; ISSN 0305-1048; ISSN NARHAD
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
ALLOCATIONS
CATIONS
CRYSTAL STRUCTURE
CRYSTALLIZATION
DYNAMICS
INFORMATION
IONS
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
PROGRAMMING LANGUAGES
TELOMERES
THALLIUM
THYMINE
national synchrotron light source