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X-ray characterization of mesophases of human telomeric G-quadruplexes and other DNA analogues

Journal Article · · Scientific Reports
DOI:https://doi.org/10.1038/srep27079· OSTI ID:1280920
 [1];  [2];  [3];  [4];  [5];  [3];  [6]
  1. Univ. of Massachusetts, Amherst, MA (United States). Dept. of Physics; Univ. of Massachusetts, Amherst, MA (United States). Dept. of Physics
  2. Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Macromolecular Science and Engineering
  3. Univ. of Massachusetts, Amherst, MA (United States). Dept. of Physics
  4. Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Macromolecular Science and Engineering, Dept. of Biomedical Engineering, Dept. of Radiology, Dept. of Materials Science and Engineering, Comprehensive Cancer Center, Div. of General Medical Sciences and Oncology
  5. Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Macromolecular Science and Engineering, Dept. of Biomedical Engineering, Dept. of Materials Science and Engineering, Dept. of Physics
  6. Univ. of Massachusetts, Amherst, MA (United States). Dept. of Physics; Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Materials Science and Engineering; J. Stefan Institute, Ljubljana (Slovenia). Dept. of Theoretical Physics; Univ. of Ljubljana, Ljubljana (Slovenia). Dept. of Physics, Faculty of Mathematics and Physics
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We report that observed in the folds of guanine-rich oligonucleotides, non-canonical G-quadruplex structures are based on G-quartets formed by hydrogen bonding and cation-coordination of guanosines. In dilute 5'-guanosine monophosphate (GMP) solutions, G-quartets form by the self-assembly of four GMP nucleotides. We use x-ray diffraction to characterize the columnar liquid-crystalline mesophases in concentrated solutions of various model G-quadruplexes. We then probe the transitions between mesophases by varying the PEG solution osmotic pressure, thus mimicking in vivo molecular crowding conditions. Using the GMP-quadruplex, built by the stacking of G-quartets with no covalent linking between them, as the baseline, we report the liquid-crystalline phase behaviors of two other related G-quadruplexes: (i) the intramolecular parallel-stranded G-quadruplex formed by the 22-mer four-repeat human telomeric sequence AG3 (TTAG3)3 and (ii) the intermolecular parallel-stranded G-quadruplex formed by the TG(4)T oligonucleotides. Finally, we compare the mesophases of the G-quadruplexes, under PEG-induced crowding conditions, with the corresponding mesophases of the canonical duplex and triplex DNA analogues.
Research Organization:
10.1038/srep27079
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22), Division of Materials Sciences and Engineering
Grant/Contract Number:
SC0008068; SC0008176
OSTI ID:
1280920
Journal Information:
Scientific Reports, Journal Name: Scientific Reports Vol. 6; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

Molecular Dynamics Simulation of High Density DNA Arrays journal January 2018

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Related Subjects

60 APPLIED LIFE SCIENCES
condensation
crystal-structure
diffraction
guanine tetraplex
helical parameters
liquid-crystals
orientational order
triplex