Direct NMR detection of the 'invisible' alkali metal cations tightly bound to G-quadruplex structures

Journal Article · Fri Nov 11 04:00:00 EST 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.08.275· OSTI ID:20713438

Wong, Alan ^[1]; Ida, Ramsey ^[1]; Gang, Wu ^[1]

---

1. Department of Chemistry, Queen's University, Kingston, Ont., K7L 3N6 (Canada)

We report the first direct solution NMR detection of the alkali metal cations ({sup 23}Na{sup +}, {sup 39}K{sup +}, and {sup 87}Rb{sup +}) residing inside G-quadruplex channel structures formed by guanosine 5'-monophosphate and a DNA oligomer, d(TG{sub 4}T). In solution, these channel alkali metal cations are tightly bound to the G-quadruplex structure and have been considered to be 'invisible' to NMR spectroscopy for many years. Our finding that it is possible to directly observe these alkali metal cations by NMR spectroscopy provides a new tool for studying cation binding affinity and dynamics in G-quadruplex DNA.

• OSTI ID: 20713438
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 337; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AFFINITY
ALKALI METALS
CATIONS
DNA
GUANOSINE
NUCLEAR MAGNETIC RESONANCE
SPECTROSCOPY