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Title: Biological Imaging by Soft X-ray Diffraction Microscopy

Abstract

We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source (NSLS)
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
913688
Report Number(s):
BNL-78256-2007-JA
Journal ID: ISSN 0027-8424; PNASA6; TRN: US200804%%35
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 102; Journal Issue: 43; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; X-RAY DIFFRACTION; YEASTS; SOFT X RADIATION; SPATIAL RESOLUTION; national synchrotron light source

Citation Formats

Shapiro, D, Thibault, P, Beetz, T, Elser, V, Howells, M, Jacobsen, C, Kirz, J, Lima, E, Miao, H, and et al. Biological Imaging by Soft X-ray Diffraction Microscopy. United States: N. p., 2005. Web. doi:10.1073/pnas.0503305102.
Shapiro, D, Thibault, P, Beetz, T, Elser, V, Howells, M, Jacobsen, C, Kirz, J, Lima, E, Miao, H, & et al. Biological Imaging by Soft X-ray Diffraction Microscopy. United States. https://doi.org/10.1073/pnas.0503305102
Shapiro, D, Thibault, P, Beetz, T, Elser, V, Howells, M, Jacobsen, C, Kirz, J, Lima, E, Miao, H, and et al. 2005. "Biological Imaging by Soft X-ray Diffraction Microscopy". United States. https://doi.org/10.1073/pnas.0503305102.
@article{osti_913688,
title = {Biological Imaging by Soft X-ray Diffraction Microscopy},
author = {Shapiro, D and Thibault, P and Beetz, T and Elser, V and Howells, M and Jacobsen, C and Kirz, J and Lima, E and Miao, H and et al.},
abstractNote = {We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.},
doi = {10.1073/pnas.0503305102},
url = {https://www.osti.gov/biblio/913688}, journal = {Proceedings of the National Academy of Sciences of the United States of America},
issn = {0027-8424},
number = 43,
volume = 102,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2005},
month = {Sat Jan 01 00:00:00 EST 2005}
}

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