Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell

Huang, Xiaojing; Nelson, Johanna; Kirz, Janos; Lima, Enju; Marchesini, Stefano; Miao, Huijie; Neiman, Aaron M.; Shapiro, David; Steinbrener, Jan; Stewart, Andrew; Turner, Joshua J.; Jacobsen, Chris

doi:10.1103/PhysRevLett.103.198101

Title: Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell

Journal Article · Sun Nov 01 00:00:00 EDT 2009 · Physical Review Letters

DOI:https://doi.org/10.1103/PhysRevLett.103.198101· OSTI ID:1164142

Huang, Xiaojing ^[1]; Nelson, Johanna ^[1]; Kirz, Janos ^[1]; Lima, Enju ^[1]; Marchesini, Stefano ^[2]; Miao, Huijie ^[1]; Neiman, Aaron M. ^[3]; Shapiro, David ^[2]; Steinbrener, Jan ^[1]; Stewart, Andrew ^[1]; Turner, Joshua J. ^[1]; Jacobsen, Chris ^[1]

Stony Brook Univ., NY (United States) Dept. of Physics and Astronomy
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Stony Brook Univ., NY (United States) Dept. of Biochemistry and Cell Biology

We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.

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Research Organization:: Stony Brook Univ., NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-04ER46128

OSTI ID:: 1164142

Journal Information:: Physical Review Letters, Vol. 103, Issue 19; ISSN 0031-9007

Publisher:: American Physical Society (APS)Copyright Statement

Country of Publication:: United States

Language:: English

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