High-resolution x-ray diffraction microscopy of specifically labeled yeast cells

Nelson, Johanna; Huang, Xiaojing; Steinbrener, Jan; Shapiro, David; Kirz, Janos; Marchesini, Stephano; Neiman, Aaron M.; Turner, Joshua J.; Jacobsen, Chris

doi:10.1073/pnas.0910874107

Title: High-resolution x-ray diffraction microscopy of specifically labeled yeast cells

Journal Article · Tue Apr 20 00:00:00 EDT 2010 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.0910874107· OSTI ID:1164150

Nelson, Johanna ^[1]; Huang, Xiaojing ^[1]; Steinbrener, Jan ^[1]; Shapiro, David ^[2]; Kirz, Janos ^[2]; Marchesini, Stephano ^[2]; Neiman, Aaron M. ^[3]; Turner, Joshua J. ^[1]; Jacobsen, Chris ^[4]

Stony Brook Univ., Stony Brook, NY (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
Northwestern Univ., Evanston, IL (United States)
Stony Brook Univ., Stony Brook, NY (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source; Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source

X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific gold labeling at different depths within cells via through-focus propagation of the reconstructed wavefield. The lectin concanavalin A conjugated to colloidal gold particles was used to label the α-mannan sugar in the cell wall of the yeast Saccharomyces cerevisiae. Cells were plunge-frozen in liquid ethane and freeze-dried, after which they were imaged whole using x-ray diffraction microscopy at 750 eV photon energy.

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Research Organization:: Stony Brook Univ., NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-04ER46128

OSTI ID:: 1164150

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 16; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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