Nucleotide sequence of a small cryptic plasmid from Acidithiobacillus ferrooxidans strain A-6

Journal Article · Wed Oct 01 04:00:00 EDT 2003 · Hydrometallurgy

DOI:https://doi.org/10.1016/S0304-386X(03)00149-X· OSTI ID:912324

Roberto, F

A 2.1 kb cryptic plasmid from Acidithiobacillus ferrooxidans strain A-6 was isolated and cloned into the E. coli vector plasmid, pUC128. The cloned plasmid was mapped by restriction enzyme fragment analysis and subsequently sequenced. At this time over half the plasmid sequence has been determined and compared to sequences in the GenBank nucleotide and protein sequence databases. Much of the plasmid remains cryptic, but substantial nucleotide and protein sequence similarities have been observed to the putative replication protein, RepA, of the small cryptic plasmids pAYS and pAYL found in the ammonia-oxidizing Nitrosomonas sp. Strain ENI-11. These results suggest an entirely new class of plasmid is maintained in at least one strain of Acidithiobacillus ferrooxidans and other acidophilic bacteria, and raises interesting questions about the origin of this plasmid in acidic environments.

• Research Organization: Idaho National Laboratory (INL)
• Sponsoring Organization: DOE - SC
• DOE Contract Number: AC07-99ID13727
• OSTI ID: 912324
• Report Number(s): INEEL/JOU-03-01492
• Journal Information: Hydrometallurgy, Journal Name: Hydrometallurgy Journal Issue: 1 - 2 Vol. 71; ISSN 0304-386X; ISSN HYDRDA
• Country of Publication: United States
• Language: English

Related Subjects

99 GENERAL AND MISCELLANEOUS
Acidithiobacillus ferrooxidans
BACTERIA
DNA sequence
ENZYMES
NUCLEOTIDES
ORIGIN
PLASMIDS
PROTEINS
STRAINS
VECTORS
pAYS homology
plasmid
repA