Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions
Journal Article
·
· Journal of the American Chemical Society, 129:8682-8683
Covalent immobilization of peptides on solid supports plays an important role in biochemistry with applications ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins to development of novel biosensors and substrates for improved cell adhesion. Self-assembled monolayer surfaces (SAMs) provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. Existing techniques for linking peptides to SAMs are based on solution-phase synthetic strategies and require relatively large quantities of purified material. Here, we report a novel approach for highly selective covalent binding of peptides to SAMs using soft-landing (SL) of mass-selected ions. SL is defined as intact deposition of ions onto suitable substrates at hyperthermal (<100 eV) energies.Recent studies have demonstrated that SAMs are excellent deposition targets for SL due to their ability to dissipate kinetic energies of the projectiles and their efficiency in trapping captured species. It has been proposed that SL could be utilized for controlled preparation of protein arrays.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 910002
- Report Number(s):
- PNNL-SA-54400; 16731; 19594; KC0302020
- Journal Information:
- Journal of the American Chemical Society, 129:8682-8683, Journal Name: Journal of the American Chemical Society, 129:8682-8683 Vol. 129; ISSN JACSAT; ISSN 0002-7863
- Country of Publication:
- United States
- Language:
- English
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