Reactive Landing of Peptide Ions on Self-Assembled Monolayer Surfaces: A Alternative Approach for Covalent Immobilization of Peptides on Surfaces

Journal Article · Tue Jan 01 04:00:00 EST 2008 · Physical Chemistry Chemical Physics. PCCP, 10(11):1512-1522

DOI:https://doi.org/10.1039/b717617a· OSTI ID:927490

Wang, Peng; Hadjar, Omar; Gassman, Paul L; Laskin, Julia

Soft landing of mass-selected peptide ions onto reactive self-assembled monolayer surfaces (SAMs) was performed using a newly constructed ion deposition apparatus. SAM surfaces before and after soft-landing were characterized ex situ using time of flight-secondary ion mass spectrometry (TOF-SIMS) and infrared reflection absorption spectroscopy (IRRAS). We demonstrate that reactive landing (RL) results in efficient covalent linking of lysine-containing peptides onto the SAM of N-hydroxysuccinimidyl ester terminated alkylthiol on gold (NHS-SAM). Systematic studies of the factors that affect the efficiency of RL revealed that the reaction takes place upon collision and is promoted by the kinetic energy of the ion. The efficiency of RL maximizes at ca. 40 eV collision energy. At high collision energies the RL efficiency decreases because of the competition with scattering of ions off the surface. The reaction yield is independent of the charge state of the projectile ions suggesting that peptide ions undergo efficient neutralization upon collision. Chemical and physical properties of the SAM surface are also important factors that affect the outcome of RL. The presence of chemically reactive functional groups on the SAM surface significantly improves the reaction efficiency. RL of mass- and energy-selected peptide ions on surfaces provides a highly specific approach for covalent immobilization of biological molecules onto SAM surfaces.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 927490
• Report Number(s): PNNL-SA-56290; 19594; 16731; KC0302020
• Journal Information: Physical Chemistry Chemical Physics. PCCP, 10(11):1512-1522, Journal Name: Physical Chemistry Chemical Physics. PCCP, 10(11):1512-1522 Journal Issue: 11 Vol. 10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COVALENCE
DEPOSITION
Environmental Molecular Sciences Laboratory
IMMOBILIZED CELLS
IONS
PEPTIDES
SURFACES