Method for selective immobilization of macromolecules on self assembled monolayer surfaces

Laskin, Julia; Wang, Peng

Method for selective immobilization of macromolecules on self assembled monolayer surfaces

Patent · 28 November 2011

OSTI ID:1032634

Laskin, Julia ^[1]; Wang, Peng ^[2]

Richland, WA
Billerica, MA

Disclosed is a method for selective chemical binding and immobilization of macromolecules on solid supports in conjunction with self-assembled monolayer (SAM) surfaces. Immobilization involves selective binding of peptides and other macromolecules to SAM surfaces using reactive landing (RL) of mass-selected, gas phase ions. SAM surfaces provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. The invention finds applications in biochemistry ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins, to development of novel biosensors and substrates for stimulated protein and cell adhesion.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC06-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 8,067,053

Application Number:: 12/151,945

OSTI ID:: 1032634

Country of Publication:: United States

Language:: English

References (13)

Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview Laskin, Julia; Wang, Peng; Hadjar, Omar Phys. Chem. Chem. Phys., Vol. 10, Issue 8 https://doi.org/10.1039/B712710C	journal	January 2008
Ion/surface reactions and ion soft-landing Gologan, Bogdan; Green, Jason R.; Alvarez, Jormarie Physical Chemistry Chemical Physics, Vol. 7, Issue 7 https://doi.org/10.1039/b418056a	journal	January 2005
Design and Performance of an Instrument for Soft Landing of Biomolecular Ions on Surfaces Hadjar, Omar; Wang, Peng; Futrell, Jean H. Analytical Chemistry, Vol. 79, Issue 17 https://doi.org/10.1021/ac070600h	journal	September 2007
Preparative Soft and Reactive Landing of Gas-Phase Ions on Plasma-Treated Metal Surfaces Volný, Michael; Elam, W. Timothy; Ratner, Buddy D. Analytical Chemistry, Vol. 77, Issue 15 https://doi.org/10.1021/ac0505019	journal	August 2005
Covalent immobilization of native biomolecules onto Au(111) via N-hydroxysuccinimide ester functionalized self-assembled monolayers for scanning probe microscopy Wagner, P.; Hegner, M.; Kernen, P. Biophysical Journal, Vol. 70, Issue 5 https://doi.org/10.1016/S0006-3495(96)79810-7	journal	May 1996
Charge retention by peptide ions soft-landed onto self-assembled monolayer surfaces Laskin, Julia; Wang, Peng; Hadjar, Omar International Journal of Mass Spectrometry, Vol. 265, Issue 2-3 https://doi.org/10.1016/j.ijms.2007.02.013	journal	September 2007
Soft-Landing of Peptides onto Self-Assembled Monolayer Surfaces ^† Alvarez, Jormarie; Futrell, Jean H.; Laskin, Julia The Journal of Physical Chemistry A, Vol. 110, Issue 4 https://doi.org/10.1021/jp0555044	journal	February 2006
Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces Wang, Peng; Hadjar, Omar; Gassman, Paul L. Physical Chemistry Chemical Physics, Vol. 10, Issue 11 https://doi.org/10.1039/b717617a	journal	January 2008
First Observation of Charge Reduction and Desorption Kinetics of Multiply Protonated Peptides Soft Landed onto Self-Assembled Monolayer Surfaces Hadjar, Omar; Futrell, Jean H.; Laskin, Julia The Journal of Physical Chemistry C, Vol. 111, Issue 49 https://doi.org/10.1021/jp075293y	journal	December 2007
Preparative Soft and Reactive Landing of Multiply Charged Protein Ions on a Plasma-Treated Metal Surface Volný, Michael; Elam, W. Timothy; Branca, Andrew Analytical Chemistry, Vol. 77, Issue 15, p. 4890-4896 https://doi.org/10.1021/ac0507136	journal	August 2005
A Strategy for the Generation of Surfaces Presenting Ligands for Studies of Binding Based on an Active Ester as a Common Reactive Intermediate: A Surface Plasmon Resonance Study Lahiri, Joydeep; Isaacs, Lyle; Tien, Joe Analytical Chemistry, Vol. 71, Issue 4 https://doi.org/10.1021/ac980959t	journal	February 1999
Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions Wang, Peng; Hadjar, Omar; Laskin, Julia Journal of the American Chemical Society, Vol. 129, Issue 28 https://doi.org/10.1021/ja071804i	journal	July 2007
Preparation and in Situ Characterization of Surfaces Using Soft Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer Alvarez, Jormarie; Cooks, R. Graham; Barlow, S. E. Analytical Chemistry, Vol. 77, Issue 11 https://doi.org/10.1021/ac0481349	journal	June 2005

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