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Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics

Patent ·
OSTI ID:874841
 [1]
  1. Decatur, GA
+ Show Author Affiliations

PCR (polymerase chain reaction) primers for the detection of certain Bacillus species, such as Bacillus anthracis. The primers specifically amplify only DNA found in the target species and can distinguish closely related species. Species-specific PCR primers for Bacillus anthracis, Bacillus globigii and Clostridium perfringens are disclosed. The primers are directed to unique sequences within sasp (small acid soluble protein) genes.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
DOE Contract Number:
AC03-76SF00098
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
US 6472155
OSTI ID:
874841
Country of Publication:
United States
Language:
English

References (2)

Cloning and nucleotide sequence of three genes coding for small, acid-soluble proteins of Clostridium perfringens spores journal January 1991
Cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus". journal January 1987

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Related Subjects

/435/999/
acid
acid-soluble
amplification
amplify
anthracis
bacillus
based
chain
chain reaction
closely
clostridium
coat
detection
diagnostics
directed
disclosed
distinguish
dna
found
genes
globigii
identification
microbes
pcr
perfringens
polymerase
primers
protein
proteins
reaction
related
sasp
sequence
sequences
soluble
species
species-specific
specific
specifically
spore
spore-producing
target
unique