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Title: Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics

Abstract

PCR (polymerase chain reaction) primers for the detection of certain Bacillus species, such as Bacillus anthracis. The primers specifically amplify only DNA found in the target species and can distinguish closely related species. Species-specific PCR primers for Bacillus anthracis, Bacillus globigii and Clostridium perfringens are disclosed. The primers are directed to unique sequences within sasp (small acid soluble protein) genes.

Inventors:
 [1]
  1. (Decatur, GA)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
OSTI Identifier:
874841
Patent Number(s):
US 6472155
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
species; specific; identification; spore-producing; microbes; sequence; acid-soluble; spore; coat; proteins; amplification; based; diagnostics; pcr; polymerase; chain; reaction; primers; detection; bacillus; anthracis; specifically; amplify; dna; found; target; distinguish; closely; related; species-specific; globigii; clostridium; perfringens; disclosed; directed; unique; sequences; sasp; acid; soluble; protein; genes; chain reaction; /435/999/

Citation Formats

McKinney, Nancy. Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics. United States: N. p., 2002. Web.
McKinney, Nancy. Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics. United States.
McKinney, Nancy. Tue . "Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics". United States. https://www.osti.gov/servlets/purl/874841.
@article{osti_874841,
title = {Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics},
author = {McKinney, Nancy},
abstractNote = {PCR (polymerase chain reaction) primers for the detection of certain Bacillus species, such as Bacillus anthracis. The primers specifically amplify only DNA found in the target species and can distinguish closely related species. Species-specific PCR primers for Bacillus anthracis, Bacillus globigii and Clostridium perfringens are disclosed. The primers are directed to unique sequences within sasp (small acid soluble protein) genes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

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