Molecular cytogenetics by polymerase catalyzed amplification or in situ labelling of specific nucleic acid sequences
- Univ. of Aarhus (Denmark)
The Polymerase Chain Reaction (PCR) can be performed on isolated cells or chromosomes and the product can be analyzed by DNA technology or by FISH to test metaphases. The authors have good experiences analyzing aberrant chromosomes by FACS sorting, PCR with degenerated primers and painting of test metaphases with the PCR product. They also utilize polymerases for PRimed IN Situ labelling (PRINS) of specific nucleic acid sequences. In PRINS oligonucleotides are hybridized to their target sequences and labeled nucleotides are incorporated at the site of hybridization with the oligonucleotide as primer. PRINS may eventually allow the study of individual genes, gene expression and even somatic mutations (in mRNA) in single cells.
- OSTI ID:
- 6555245
- Report Number(s):
- CONF-9303114--
- Journal Information:
- Cytometry (Baltimore); (United States), Journal Name: Cytometry (Baltimore); (United States) Vol. 6; ISSN CYTODQ; ISSN 0196-4763
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMPLIFICATION
CHROMOSOME SORTING
CYTOLOGICAL TECHNIQUES
DETECTION
DNA
DNA HYBRIDIZATION
ENZYMES
FLUORESCENCE
GENES
HYBRIDIZATION
LABELLING
LUMINESCENCE
MUTATIONS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
TRANSFERASES