Localization of the human gene for [mu]-crystallin to chromosome 16p
- Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States)
- Adelaide Children's Hospital, North Adelaide (Australia)
- National Eye Institute, Bethesday, MD (United States)
The mapping of the human [mu]-crystallin gene was accomplished using polymerase chain reaction (PCR) amplification of a sequence tagged site (STS) in somatic cell hybrids. Two oligonucleotides from the 3[prime]-untranslated region were synthesized and used as primers for the amplification of a 260-bp DNA sequence from genomic DNA. For localization to a specific chromosome, a commercially available panel of human-rodent somatic cell hybrids, panel 2 from NIGMS (Camden, NJ), was used. Amplification of the appropriate size STS was observed only in somatic cell hybrid GM/NA10567, which contained human chromosome 16 as the only human chromosomal material. To further define the mapping position of the [mu]-crystallin gene on human chromosome 16, DNA from another panel of mouse-human somatic cell hybrids containing portions of human chromosome 16 was used. After PCR amplification the results were consistent with localization of the [mu]-crystallin gene on the short arm of chromosome 16 between breakpoints from hybride CY175 and CY145(D)-CY13-CY123 on chromosomal bands p13.11-p12.3. 5 refs., 1 fig.
- DOE Contract Number:
- FG02-89ER60863
- OSTI ID:
- 6758581
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 14:4; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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