Smooth muscle myosin heavy chain locus (MYH11) maps to 16p13. 13-p13. 12 and establishes a new region of conserved synteny between human 16p and mouse 16
- Univ. of Texas M.D. Anderson Cancer Center, Houston, TX (United States)
- Howard Hughes Medical Institute, Cold Spring Harbor, NY (United States)
- Howard Hughes Medical Institute, Cold Spring Harbor, NY (United States) Univ. of Michigan Medical Center, Ann Arbor, MI (United States)
- Adelaide Children's Hospital (Australia)
The human smooth muscle myosin heavy chain locus (MYH11) was mapped by fluorescence in situ hybridization to the middle of the p arm of chromosome 16 using a genomic cosmid clone containing coding sequences of the gene as probe. Probe from coding sequence, when applied to Southern blots of a panel of hybrids containing different portions of human chromosome 16, localized the gene to 16p13.13-13.12. Coding sequence PCR primers, when used on the DNA from a CHO-mouse hybrid clone mapping panel informative for mouse chromosomes, showed that the gene was located on mouse chromosome 16. These results correct a recent assignment of MYH11 from 16q12.2 to the region of the 16p-arm inversion breakpoint seen in acute myelomonocytic leukemia (AMML) M4Eo and demonstrate that the conflicting data do not result from the presence of additional MYH genes on the q arm of the chromosome. Also, a new region of conserved synteny between human 16p and mouse 16 is established. 10 refs., 2 figs., 1 tab.
- DOE Contract Number:
- FG02-89ER60863
- OSTI ID:
- 6975608
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:1; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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