Correlation of physical and genetic maps of human chromosome 16
This project aims to construct a detailed physical map of human chromosome 16, which will be correlated with the linkage map of this chromosome. The methods to be used to achieve this include the development of a panel of mouse/human hybrid cell lines which contain only parts of chromosome 16. This will be achieved by fusing human cells which have rearrangements of chromosome 16 with mouse A9 cells and selecting for the human APRT gene on the end of the long arm of chromosome 16. The cytogenetic and molecular characterization of the panel will allow detailed physical mapping of cloned DNA sequences and genes which are expressed in the hybrid cells. The molecular characterization will be by Southern analysis and in situ hybridization. The cell panel produced should divide this chromosome which contains approximately 3.3% of the human genome into about 50 intervals of average size 2 mb and thus provide a means of mapping any cloned DNA sequence from chromosome 16 into these relatively small regions. Sequences which map into such a region should then be able to be used to generate restriction maps using pulse field gel electrophoresis. This project should lay the foundation for the construction of a restriction map of chromosome 16. 1 tab.
- Research Organization:
- Adelaide Children's Hospital, North Adelaide (Australia)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG02-89ER60863
- OSTI ID:
- 5601648
- Report Number(s):
- DOE/ER/60863-1; ON: DE90000146
- Country of Publication:
- United States
- Language:
- English
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