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Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ

Patent ·
OSTI ID:874041
 [1];  [2]
  1. San Francisco, CA
  2. Oakland, CA
+ Show Author Affiliations

A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 6300066
OSTI ID:
874041
Country of Publication:
United States
Language:
English

References (2)

A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides journal January 1986
Book notices journal October 1987

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acid
acid probe
allowing
amplification
autosomal
binding
chromosome
chromosome painting
chromosome specific
chromosomes
described
determining
dna
dna chromosome
highly
highly repeating
inventive
inventive primers
method
nonspecific
nonspecific binding
nucleic
nucleic acid
painting
painting sequences
pcr
primers
primers allowing
probe
probe production
probe selected
producing
production
provided
repeating
repeating sequences
sample
sample amplification
selected
sequences
signal
situ
specific
specific binding
specific nucleic
specific probe