Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ
Patent
·
OSTI ID:321197
A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences. 9 figs.
- Research Organization:
- University of California
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- Univ. of California, Alameda, CA (United States)
- Patent Number(s):
- US 5,840,482/A/
- Application Number:
- PAN: 7-594,921
- OSTI ID:
- 321197
- Country of Publication:
- United States
- Language:
- English
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