Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU
Patent
·
OSTI ID:872226
- Livermore, CA
- Tracy, CA
A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Number(s):
- US 5888730
- OSTI ID:
- 872226
- Country of Publication:
- United States
- Language:
- English
A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides
|
journal | January 1986 |
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chromosome specific
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dna
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pcr
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sample
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selected
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situ
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acid
acid probe
allowing
amplification
autosomal
binding
chromosome
chromosome painting
chromosome specific
chromosomes
described
dna
dna chromosome
highly
highly repeating
identifying
inventive
inventive primers
method
nonspecific
nonspecific binding
nucleic
nucleic acid
painting
painting sequences
pcr
primers
primers allowing
probe
probe production
probe selected
producing
production
provided
repeating
repeating sequences
sample
sample amplification
selected
sequences
signal
situ
specific
specific binding
specific nucleic
specific probe