Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU
Patent
·
OSTI ID:335464
A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences. 9 figs.
- Research Organization:
- Univ. of California (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- Univ. of California, Oakland, CA (United States)
- Patent Number(s):
- US 5,888,730/A/
- Application Number:
- PAN: 8-546,452
- OSTI ID:
- 335464
- Resource Relation:
- Other Information: PBD: 30 Mar 1999
- Country of Publication:
- United States
- Language:
- English
A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides
|
journal | January 1986 |
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