Dyes designed for high sensitivity detection of double-stranded DNA

Glazer, Alexander N; Benson, Scott C

Dyes designed for high sensitivity detection of double-stranded DNA

Patent · Fri Dec 31 23:00:00 EST 1999

OSTI ID:872967

Glazer, Alexander N ^[1]; Benson, Scott C ^[2]

Orinda, CA
Oakland, CA

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

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Research Organization:: University of California

DOE Contract Number:: FG03-91ER61125

Assignee:: Regents of University of California (Berkeley, CA)

Patent Number(s):: US 6054272

OSTI ID:: 872967

Country of Publication:: United States

Language:: English

References (22)

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Dyes designed for high sensitivity detection of double-stranded DNA

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