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Dyes designed for high sensitivity detection of double-stranded DNA

Patent ·
OSTI ID:869304
 [1];  [2]
  1. Orinda, CA
  2. Albany, CA
+ Show Author Affiliations

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Research Organization:
University of California
DOE Contract Number:
FG03-91ER61125
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5312921
OSTI ID:
869304
Country of Publication:
United States
Language:
English

References (10)

Capillary electrophoresis with laser-induced fluorescence detection of PCR fragments using thiazole orange journal August 1992
Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing journal September 1992
Excitation energy dependence of intramolecular excimer formation in N,N,N',N'-tetramethylpropanediamine vapor journal February 1983
Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications journal January 1992
Color and Constitution. V. 1 The Absorption of Unsymmetrical Cyanines. Resonance as a Basis for a Classification of Dyes journal February 1942
DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligands journal November 1978
DNA bifunctional intercalators. 1. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer journal November 1978
High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange journal January 1991
Thiazole orange: A new dye for reticulocyte analysis journal November 1986
Color and Constitution. II. 1 Absorptions of Some Related Vinylene-Homologous Series * journal November 1941

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Related Subjects

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