Dyes designed for high sensitivity detection of double-stranded DNA
Abstract
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
- Inventors:
-
- Orinda, CA
- Albany, CA
- Publication Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 869304
- Patent Number(s):
- US 5312921
- Assignee:
- Regents of University of California (Oakland, CA)
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dyes; designed; sensitivity; detection; double-stranded; dna; novel; fluorescent; provided; characterized; fluorophore; joined; polycationic; chain; positive; charges; found; provide; enhancement; binding; nucleic; acid; strong; affinities; compared; dsdna; electrophoresis; solution; substantially; sensitivities; dye; novel fluorescent; stranded dna; dyes designed; double-stranded dna; nucleic acid; fluorescent dyes; fluorescent dye; sensitivity detection; strong binding; binding affinities; polycationic chain; cationic chain; fluorophore joined; /999/
Citation Formats
Glazer, Alexander N, and Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States: N. p., 1994.
Web.
Glazer, Alexander N, & Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States.
Glazer, Alexander N, and Benson, Scott C. 1994.
"Dyes designed for high sensitivity detection of double-stranded DNA". United States. https://www.osti.gov/servlets/purl/869304.
@article{osti_869304,
title = {Dyes designed for high sensitivity detection of double-stranded DNA},
author = {Glazer, Alexander N and Benson, Scott C},
abstractNote = {Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.},
doi = {},
url = {https://www.osti.gov/biblio/869304},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1994},
month = {Sat Jan 01 00:00:00 EST 1994}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
DNA bifunctional intercalators. 1. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer
journal, November 1978
- Gaugain, Bernard; Barbet, Jacques; Oberlin, Robert
- Biochemistry, Vol. 17, Issue 24
DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligands
journal, November 1978
- Gaugain, Bernard; Barbet, Jacques; Capelle, Nicole
- Biochemistry, Vol. 17, Issue 24
Color and Constitution. V. 1 The Absorption of Unsymmetrical Cyanines. Resonance as a Basis for a Classification of Dyes
journal, February 1942
- Brooker, L. G. S.; Keyes, G. H.; Williams, W. W.
- Journal of the American Chemical Society, Vol. 64, Issue 2
Excitation energy dependence of intramolecular excimer formation in N,N,N',N'-tetramethylpropanediamine vapor
journal, February 1983
- Itoh, Michiya; Hanashima, Yukio; Hanazaki, Ichiro
- The Journal of Physical Chemistry, Vol. 87, Issue 4
High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange
journal, January 1991
- Rye, Hays S.; Quesada, Mark A.; Peck, Konan
- Nucleic Acids Research, Vol. 19, Issue 2
Color and Constitution. II. 1 Absorptions of Some Related Vinylene-Homologous Series *
journal, November 1941
- Brooker, L. G. S.; White, F. L.; Keyes, G. H.
- Journal of the American Chemical Society, Vol. 63, Issue 11
Thiazole orange: A new dye for reticulocyte analysis
journal, November 1986
- Lee, Linda G.; Chen, Chia-Huei; Chiu, Laura A.
- Cytometry, Vol. 7, Issue 6
Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing
journal, September 1992
- Mathies, Richard A.; Huang, Xiaohua C.
- Nature, Vol. 359, Issue 6391
Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications
journal, January 1992
- Rye, Hays S.; Yue, Stephen; Wemmer, David E.
- Nucleic Acids Research, Vol. 20, Issue 11
Capillary electrophoresis with laser-induced fluorescence detection of PCR fragments using thiazole orange
journal, August 1992
- Schwartz, Herbert E.; Ulfelder, Kathi J.
- Analytical Chemistry, Vol. 64, Issue 15