Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

Kwok, Pui-Yan; Chen, Xiangning

Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

Patent · Fri Jan 01 04:00:00 EST 1999

OSTI ID:872487

Kwok, Pui-Yan ^[1]; Chen, Xiangning ^[2]

Clayton, MO
St. Louis, MO

A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

Research Organization:: University of Washington

DOE Contract Number:: FG06-94ER61909

Assignee:: Washington University (St. Louis, MO)

Patent Number(s):: US 5945283

OSTI ID:: 872487

Country of Publication:: United States

Language:: English

References (11)

Allelic discrimination by nick-translation PCR with fluorgenic probes Lee, Linda G.; Connell, Charles R.; Bloch, Will Nucleic Acids Research, Vol. 21, Issue 16, p. 3761-3766 https://doi.org/10.1093/nar/21.16.3761	journal	August 1993
Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms Nikiforov, Theo T.; Rendie, Robert B.; Goelet, Philip Nucleic Acids Research, Vol. 22, Issue 20 https://doi.org/10.1093/nar/22.20.4167	journal	January 1994
A novel method for real time quantitative RT-PCR. Gibson, U. E.; Heid, C. A.; Williams, P. M. Genome Research, Vol. 6, Issue 10 https://doi.org/10.1101/gr.6.10.995	journal	October 1996
Molecular Beacons: Probes that Fluoresce upon Hybridization Tyagi, Sanjay; Kramer, Fred Russell Nature Biotechnology, Vol. 14, Issue 3, p. 303-308 https://doi.org/10.1038/nbt0396-303	journal	March 1996
Real time quantitative PCR. Heid, C. A.; Stevens, J.; Livak, K. J. Genome Research, Vol. 6, Issue 10, p. 986-994 https://doi.org/10.1101/gr.6.10.986	journal	October 1996
Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Holland, P. M.; Abramson, R. D.; Watson, R. Proceedings of the National Academy of Sciences, Vol. 88, Issue 16, p. 7276-7280 https://doi.org/10.1073/pnas.88.16.7276	journal	August 1991
Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. Cardullo, R. A.; Agrawal, S.; Flores, C. Proceedings of the National Academy of Sciences, Vol. 85, Issue 23 https://doi.org/10.1073/pnas.85.23.8790	journal	December 1988
A ligase-mediated gene detection technique Landegren, U.; Kaiser, R.; Sanders, J. Science, Vol. 241, Issue 4869 https://doi.org/10.1126/science.3413476	journal	August 1988
Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay. Nickerson, D. A.; Kaiser, R.; Lappin, S. Proceedings of the National Academy of Sciences, Vol. 87, Issue 22 https://doi.org/10.1073/pnas.87.22.8923	journal	November 1990
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. Livak, K. J.; Flood, S. J.; Marmaro, J. Genome Research, Vol. 4, Issue 6 https://doi.org/10.1101/gr.4.6.357	journal	June 1995
DNA analysis and diagnostics on oligonucleotide microchips. Yershov, G.; Barsky, V.; Belgovskiy, A. Proceedings of the National Academy of Sciences, Vol. 93, Issue 10 https://doi.org/10.1073/pnas.93.10.4913	journal	May 1996

Similar Records

Method for promoting specific alignment of short oligonucleotides on nucleic acids

Patent · Sun Dec 31 23:00:00 EST 1995 · OSTI ID:870573

Nucleic acid sequence detection using multiplexed oligonucleotide PCR

Patent · Mon Dec 25 23:00:00 EST 2006 · OSTI ID:903353

Composition for nucleic acid sequencing

Patent · Tue Aug 26 00:00:00 EDT 2008 · OSTI ID:985535

Related Subjects

/435/436/
acid
acid target
addition
analysis
applicable
comprised
denaturation
detecting
detection
dideoxynucleoside
disclosed
doubly
energy
energy transfer
fluorescence
fluorophores
formed
forming
genotyping
indicates
kits
labeled
ligation
method
methods
nucleic
nucleic acid
nucleoside triphosphate
nucleotide
nucleotides
oligonucleotide
particularly
particularly applicable
polynucleotide
polynucleotides
presence
provided
resonance
resonance energy
sequence
singly
site
target
target nucleotide
target site
transfer
triphosphate

Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

Citation Formats

References (11)

Similar Records

Related Subjects