Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Method for promoting specific alignment of short oligonucleotides on nucleic acids

Patent ·
OSTI ID:870573
 [1];  [2];  [3]
  1. Stony Brook, NY
  2. Coram, NY
  3. Bellport, NY
+ Show Author Affiliations
Disclosed is a method for promoting specific alignment of short oligonucleotides on a nucleic acid polymer. The nucleic acid polymer is incubated in a solution containing a single-stranded DNA-binding protein and a plurality of oligonucleotides which are perfectly complementary to distinct but adjacent regions of a predetermined contiguous nucleotide sequence in the nucleic acid polymer. The plurality of oligonucleotides anneal to the nucleic acid polymer to form a contiguous region of double stranded nucleic acid. Specific application of the methods disclosed include priming DNA synthesis and template-directed ligation.
Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Number(s):
US 5547843
OSTI ID:
870573
Country of Publication:
United States
Language:
English

References (5)

Proposal for sequencing DNA using ligation of hexamers to generate sequential elongation primers (SPEL-6) journal May 1990
Sequencing of megabase plus DNA by hybridization: Theory of the method journal February 1989
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E.coli SSB journal January 1992
Improved yields of long PCR products using gene 32 protein journal January 1990
A library of 3342 useful nonamer primers for genome sequencing journal January 1990

Similar Records

Composition for nucleic acid sequencing
Patent · Tue Aug 26 00:00:00 EDT 2008 · OSTI ID:985535
Method for sequencing nucleic acid molecules
Patent · Tue May 30 00:00:00 EDT 2006 · OSTI ID:1175760
Method for sequencing nucleic acid molecules
Patent · Tue Jun 06 00:00:00 EDT 2006 · OSTI ID:1175774

Related Subjects

/435/
acid
acid polymer
acids
adjacent
alignment
anneal
application
binding protein
complementary
containing
contiguous
disclosed
distinct
dna
dna synthesis
dna-binding
double
double stranded
form
incubated
ligation
method
methods
nucleic
nucleic acid
nucleic acids
nucleotide
nucleotide sequence
oligonucleotides
perfectly
plurality
polymer
predetermined
priming
promoting
promoting specific
protein
region
regions
sequence
single-stranded
single-stranded dna
solution
solution containing
specific
specific alignment
specific application
stranded
stranded dna
stranded nucleic
synthesis
template-directed