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Title: Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates

Abstract

The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example, detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest, detecting a polypeptide such as those expressed by infectious agents, fungi or parasites.

Inventors:
 [1];  [2]
  1. Whitestone, NY
  2. New York, NY
Publication Date:
Research Org.:
University of Vermont
OSTI Identifier:
872397
Patent Number(s):
US 5925532
Assignee:
Trustees of Columbia University in City of New York (New York, NY)
DOE Contract Number:  
FG02-88ER60742
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
enhancing; sensitivity; immunoassay; procedures; antibodies; directed; product; reaction; probe; labels; assay; substrates; subject; provides; antibody; specifically; binds; labeling; substance; substrate; method; immunogen; produce; methods; detecting; antigen; sample; example; protein; comprising; amino; acid; sequence; nucleic; molecule; polypeptide; expressed; infectious; agents; fungi; parasites; acid molecule; provides methods; acid sequence; amino acid; nucleic acid; specifically bind; molecule comprising; specifically binds; immunoassay procedures; probe labels; antibodies directed; assay substrates; /435/436/

Citation Formats

Erlanger, Bernard F, and Chen, Bi-Xing. Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates. United States: N. p., 1999. Web.
Erlanger, Bernard F, & Chen, Bi-Xing. Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates. United States.
Erlanger, Bernard F, and Chen, Bi-Xing. 1999. "Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates". United States. https://www.osti.gov/servlets/purl/872397.
@article{osti_872397,
title = {Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates},
author = {Erlanger, Bernard F and Chen, Bi-Xing},
abstractNote = {The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example, detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest, detecting a polypeptide such as those expressed by infectious agents, fungi or parasites.},
doi = {},
url = {https://www.osti.gov/biblio/872397}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Works referenced in this record:

The use of tetramethylbenzidine for solid phase immunoassays
journal, December 1990


Development and Evaluation of a Microtiter Plate Enzyme Immunoassay for Antibodies to 3,3′-Dichlorobenzidine
journal, January 1987


Catalyzed reporter deposition, a novel method of signal amplification
journal, March 1991


Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays
journal, December 1989


Amplification systems in immunoenzymatic techniques
journal, June 1992


Generation of human monoclonal antibodies reactive with cellular antigens.
journal, April 1983


A strategy for immunohistochemical signal enhancement by end-product amplification.
journal, August 1996


The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats
journal, June 1992


Stepwise amplified immunoperoxidase (PAP) staining. I. Cellular morphology in relation to membrane markers.
journal, February 1984