Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates

Erlanger, Bernard F; Chen, Bi-Xing

Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates

Patent · Thu Dec 31 23:00:00 EST 1998

OSTI ID:872397

Erlanger, Bernard F ^[1]; Chen, Bi-Xing ^[2]

Whitestone, NY
New York, NY

The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example, detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest, detecting a polypeptide such as those expressed by infectious agents, fungi or parasites.

Research Organization:: University of Vermont

DOE Contract Number:: FG02-88ER60742

Assignee:: Trustees of Columbia University in City of New York (New York, NY)

Patent Number(s):: US 5925532

OSTI ID:: 872397

Country of Publication:: United States

Language:: English

References (11)

Generation of human monoclonal antibodies reactive with cellular antigens. Cote, R. J.; Morrissey, D. M.; Houghton, A. N. Proceedings of the National Academy of Sciences, Vol. 80, Issue 7 https://doi.org/10.1073/pnas.80.7.2026	journal	April 1983
Stepwise amplified immunoperoxidase (PAP) staining. I. Cellular morphology in relation to membrane markers. Lansdorp, P. M.; van der Kwast, T. H.; de Boer, M. Journal of Histochemistry & Cytochemistry, Vol. 32, Issue 2 https://doi.org/10.1177/32.2.6198353	journal	February 1984
The use of tetramethylbenzidine for solid phase immunoassays McKimm-Breschkin, Jennifer L. Journal of Immunological Methods, Vol. 135, Issue 1-2 https://doi.org/10.1016/0022-1759(90)90282-Z	journal	December 1990
Development and Evaluation of a Microtiter Plate Enzyme Immunoassay for Antibodies to 3,3′-Dichlorobenzidine Spierto, Francis W.; Vogt, Robert; Whitfield, Wanda Journal of Analytical Toxicology, Vol. 11, Issue 1 https://doi.org/10.1093/jat/11.1.31	journal	January 1987
Catalyzed reporter deposition, a novel method of signal amplification Bobrow, Mark N.; Shaughnessy, Krista J.; Litt, Gerald J. Journal of Immunological Methods, Vol. 137, Issue 1 https://doi.org/10.1016/0022-1759(91)90399-Z	journal	March 1991
Amplification systems in immunoenzymatic techniques Avrameas, S. Journal of Immunological Methods, Vol. 150, Issue 1-2 https://doi.org/10.1016/0022-1759(92)90062-X	journal	June 1992
The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats Bobrow, M. N.; Litt, G. J.; Shaughnessy, K. J. Journal of Immunological Methods, Vol. 150, Issue 1-2 https://doi.org/10.1016/0022-1759(92)90073-3	journal	June 1992
A Comparative Study of the Peroxidase-antiperoxidase Method and an Avidin-Biotin Complex Method for Studying Polypeptide Hormones with Radioimmunoassay Antibodies Hsu, Su-ming; Raine, Laurence; Fanger, Herbert American Journal of Clinical Pathology, Vol. 75, Issue 5 https://doi.org/10.1093/ajcp/75.5.734	journal	May 1981
A strategy for immunohistochemical signal enhancement by end-product amplification. Chen, B. X.; Szabolcs, M. J.; Matsushima, A. Y. Journal of Histochemistry & Cytochemistry, Vol. 44, Issue 8 https://doi.org/10.1177/44.8.8756754	journal	August 1996
Immunocytochemistry Today Van Noorden, S.; Polak, J. M. Immunocytochemistry https://doi.org/10.1016/B978-0-7236-0669-7.50009-X	book	January 1983
Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays Bobrow, Mark N.; Harris, Thomas D.; Shaughnessy, Krista J. Journal of Immunological Methods, Vol. 125, Issue 1-2 https://doi.org/10.1016/0022-1759(89)90104-X	journal	December 1989

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Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates

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References (11)

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