5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group

Pirrung, Michael C; Shuey, Steven W; Bradley, Jean-Claude

5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group

Patent · Thu Dec 31 23:00:00 EST 1998

OSTI ID:872315

Pirrung, Michael C ^[1]; Shuey, Steven W ^[2]; Bradley, Jean-Claude ^[2]

Houston, TX
Durham, NC

The present invention relates, in general, to a method of synthesizing a nucleic acid, and, in particular, to a method of effecting 5' to 3' nucleic acid synthesis. The method can be used to prepare arrays of oligomers bound to a support via their 5' end. The invention also relates to a method of effecting mutation analysis using such arrays. The invention further relates to compounds and compositions suitable for use in such methods.

Research Organization:: Duke University (Durham, NC)

DOE Contract Number:: FG05-92ER61388

Assignee:: Duke University (Durham, NC)

Patent Number(s):: US 5908926

OSTI ID:: 872315

Country of Publication:: United States

Language:: English

References (9)

Photoremovable Protecting Groups for Phosphorylation of Chiral Alcohols. Asymmetric Synthesis of Phosphotriesters of (-)-3',5'-Dimethoxybenzoin Pirrung, Michael C.; Shuey, Steven W. The Journal of Organic Chemistry, Vol. 59, Issue 14 https://doi.org/10.1021/jo00093a021	journal	July 1994
Photochemical protodesilylation of 2-R3Si-1,3-dimethoxybenzenes. Direct observation of .beta.-silyl-substituted cyclohexadienyl cations Lew, Calvin S. Q.; McClelland, Robert A. Journal of the American Chemical Society, Vol. 115, Issue 24 https://doi.org/10.1021/ja00077a058	journal	December 1993
Multiplexed biochemical assays with biological chips Fodor, Stephen P. A.; Rava, Richard P.; Huang, Xiaohua C. Nature, Vol. 364, Issue 6437 https://doi.org/10.1038/364555a0	journal	August 1993
Photochemically-removable silyl protecting groups Pirrung, Michael C.; Lee, Yong Rok The Journal of Organic Chemistry, Vol. 58, Issue 25 https://doi.org/10.1021/jo00077a010	journal	December 1993
Light-directed, spatially addressable parallel chemical synthesis Fodor, S.; Read, J.; Pirrung, M. Science, Vol. 251, Issue 4995 https://doi.org/10.1126/science.1990438	journal	February 1991
Recognition of mixed-sequence duplex DNA by alternate-strand triple-helix formation Horne, David A.; Dervan, Peter B. Journal of the American Chemical Society, Vol. 112, Issue 6 https://doi.org/10.1021/ja00162a063	journal	March 1990
Photochemistry of phosphate esters Givens, Richard S.; Kueper, L. William. Chemical Reviews, Vol. 93, Issue 1 https://doi.org/10.1021/cr00017a004	journal	January 1993
Dimethoxybenzoin Carbonates: Photochemically-Removable Alcohol Protecting Groups Suitable for Phosphoramidite-Based DNA Synthesis Pirrung, Michael C.; Bradley, Jean-Claude The Journal of Organic Chemistry, Vol. 60, Issue 5 https://doi.org/10.1021/jo00110a011	journal	March 1995
Comparison of Methods for Photochemical Phosphoramidite-Based DNA Synthesis Pirrung, Michael C.; Bradley, Jean-Claude The Journal of Organic Chemistry, Vol. 60, Issue 20 https://doi.org/10.1021/jo00125a010	journal	October 1995

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Related Subjects

-photoremovable
/999/
acid
acid synthesis
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arrays
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effecting
method
methods
mutation
nucleic
nucleic acid
oligomers
particular
prepare
protecting
relates
suitable
support
synthesis
synthesizing
via

5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group

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References (9)

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