Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages

Patent · Thu Jan 01 04:00:00 EST 1998

OSTI ID:871951

Studier, F William ^[1]; Dubendorff, John W ^[2]

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1. Stony Brook, NY
2. Sound Beach, NY

This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.

• Research Organization: Brookhaven National Laboratory (BNL), Upton, NY
• Assignee: Associated Universities, Inc. (Washington, DC)
• Patent Number(s): US 5830694
• OSTI ID: 871951
• Country of Publication: United States
• Language: English

Related Subjects

/435/
achieved
activity
autogene
autogenes
autogenes encoding
bacteriophages
cell
cell growth
cloning
combining
controlled
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encoded
encoding
encoding rna
expression
expression systems
genes encoding
growth
host
host cell
independent
inhibition
lac-repression
lysozyme
materials
methods
operator-t7
permit
polymerase
polymerases
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producing selected
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promoter
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recombinant
reducing
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relates
rna
rna poly
rna polymerase
rna polymerases
selected
sufficiently
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t7
t7 rna
t7-like
t7-like bacteriophages
transcribed